Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Biochem Biophys Res Commun. 2012 Jun 22;423(1):195-9. doi: 10.1016/j.bbrc.2012.05.119. Epub 2012 May 30.
A novel biosensor (immuno-rotary biosensor) was developed by conjugating deoxynivalenol (DON) monoclonal antibodies with the "rotator" ε-subunit of F(0)F(1)-ATPase within chromatophores with an ε-subunit monoclonal antibody-biotin-avidin-biotin linker to capture DON residues. The conjugation conditions were then optimized. The capture of DON was based on the antibody-antigen reaction and it is indicated by the change in ATP synthetic activity of F(0)F(1)-ATPase, which is measured via chemiluminescence using the luciferin-luciferase system with a computerized microplate luminometer analyzer. 10(-7)mg/ml of DON can be detected. The whole detection process requires only about 20min. This method has promising applications in the detection of small molecular compounds because of its rapidity, simplicity, and sensitivity.
一种新型生物传感器（免疫旋转生物传感器）通过将脱氧雪腐镰刀菌烯醇（DON）单克隆抗体与类囊体中的 F(0)F(1)-ATP 酶的“旋转器”ε 亚基偶联，利用 ε 亚基单克隆抗体-生物素-亲和素-生物素接头来捕获 DON 残基来开发。然后优化了偶联条件。DON 的捕获基于抗体-抗原反应，并通过使用荧光素-荧光素酶系统通过化学发光和计算机化微孔板发光计分析仪测量 F(0)F(1)-ATP 酶的 ATP 合成活性的变化来指示。可以检测到 10(-7)mg/ml 的 DON。整个检测过程仅需约 20 分钟。由于其快速、简单和灵敏，该方法在小分子化合物的检测中具有广阔的应用前景。
