Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Int J Biol Macromol. 2012 Dec;51(5):1189-95. doi: 10.1016/j.ijbiomac.2012.08.028. Epub 2012 Aug 30.
The optimal conditions for sulfation of polysaccharides from persimmon fruits (PFP) with chlorosulfonic acid-pyridine (CSA-Pyr) method were determined by response surface methodology. Box-Behnken design was applied to evaluate the effects of three independent variables (volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time) on the degree of substitution (DS), molecular weight (MW) and activated partial thromboplastin time (APTT) of sulfated polysaccharides (PFP-S). The APTT activity of PFP-S could be improved by application of various volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time, which was possible due to the degradation of polysaccharides to different extent and increasing of DS. The optimal conditions to obtain the strongest APTT of PFP-S were the volume ratio of CSA to Pyr of 1:8, the volume ratio of SO(3)Pyr to PFP of 1:3.6 and the reaction time of 3 h, respectively.
采用响应面法优化了以氯磺酸-吡啶（CSA-Pyr）法对柿果多糖（PFP）进行硫酸酯化的最佳条件。Box-Behnken 设计用于评估三个独立变量（Pyr 与 CSA 的体积比、PFP 与 SO（3）Pyr 的体积比和反应时间）对硫酸化多糖（PFP-S）取代度（DS）、分子量（MW）和活化部分凝血活酶时间（APTT）的影响。通过应用各种 Pyr 与 CSA 的体积比、PFP 与 SO（3）Pyr 的体积比和反应时间，可以提高 PFP-S 的 APTT 活性，这是由于多糖在不同程度上的降解和 DS 的增加所致。获得 PFP-S 最强 APTT 的最佳条件分别为 CSA 与 Pyr 的体积比为 1:8、SO（3）Pyr 与 PFP 的体积比为 1:3.6 和反应时间为 3 h。
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