Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2013 Jan;157(1):1-8. doi: 10.1016/j.cbpc.2012.08.003. Epub 2012 Aug 29.
We investigated the effects of acidic pH and acute aluminum (Al) exposure on the metabolic substrates of Astyanax bimaculatus, and on the ability of these animals to recover in clean water. After an acclimation period, sexually mature A. bimaculatus females were sorted into six glass aquaria with three experimental groups: control in neutral pH (7.0), acidic pH (5.5), and Al (0.5 mg·L(-1)) in acidic pH (5.5). After a 96 h treatment, 10 animals from each experimental group were sampled and the rest were returned to clean water in neutral pH without Al for a recovery period of 96 h. The acidic pH, either alone or combined with Al, decreased T4 levels, whereas Al exposure increased T3 levels. Recovery of T3 levels occurred after 96 h. Al exposure decreased ovary and plasma proteins, muscle glycogen contents, and hepatic lipids due to lipoperoxidation. In the recovery phase, lipids decreased in most tissues, probably to re-establish ovary protein and hepatic glycogen. A. bimaculatus prioritized the use of energetic resources during acclimatization to Al instead of prioritizing reproduction, thereby avoiding the ovulation of impaired eggs.
我们研究了酸性 pH 值和急性铝 (Al) 暴露对 Astyanax bimaculatus 代谢底物的影响，以及这些动物在清洁水中恢复的能力。在适应期后，将性成熟的 A. bimaculatus 雌性鱼按三种实验条件（中性 pH 值 7.0、酸性 pH 值 5.5 和酸性 pH 值 5.5 下的 0.5mg·L(-1)Al）分到六个玻璃水族箱中。经过 96 小时的处理后，从每个实验组中抽取 10 条鱼进行采样，其余鱼则返回清洁水的中性 pH 值环境中，没有 Al，进行 96 小时的恢复期。单独的酸性 pH 值或与 Al 结合都会降低 T4 水平，而 Al 暴露会增加 T3 水平。经过 96 小时的恢复，T3 水平得到了恢复。Al 暴露会降低卵巢和血浆蛋白、肌肉糖原含量以及肝脂质，这是由于脂质过氧化。在恢复阶段，大多数组织中的脂质减少，可能是为了重新建立卵巢蛋白和肝糖原。A. bimaculatus 在适应 Al 时优先利用能量资源，而不是优先繁殖，从而避免了受损卵子的排卵。
