Institute of Chemistry, University of Tartu, Ravila 14a, Tartu 50411, Estonia.
Environ Sci Pollut Res Int. 2013 Apr;20(4):2492-8. doi: 10.1007/s11356-012-1166-8. Epub 2012 Sep 8.
Although the long incubation time of biochemical oxygen demand (BOD7) measurements has been addressed by the use of microbial biosensors, the resulting sensor-BOD values gained from the measurements with specific industrial wastewaters still underestimates the BOD value of such samples. This research aims to provide fast and more accurate BOD measurements in the dairy wastewater samples. Unlike municipal wastewater, wastewater from the dairy industry contains many substrates that are not easily accessible to a majority of microorganisms. Therefore, a bacterial culture, Microbacterium phyllosphaerae, isolated from dairy wastewater was used to construct a semi-specific microbial biosensor. A universal microbial biosensor based on Pseudomonas fluorescens, which has a wide substrate spectrum but is nonspecific to dairy wastewater, was used as a comparison. BOD biosensors were calibrated with OECD synthetic wastewater, and experiments with different synthetic and actual wastewater samples were carried out. Results show that the semi-specific M. phyllosphaerae-based microbial biosensor is more sensitive towards wastewaters that contain milk derivates and butter whey than the P. fluorescens-based biosensor. Although the M. phyllosphaerae biosensor underestimates the BOD7 value of actual dairy wastewaters by 25-32%, this bacterial culture is more suitable for BOD monitoring in dairy wastewater than P. fluorescens, which underestimated the same samples by 46-61%.
虽然生化需氧量（BOD7）测量的长潜伏期可以通过使用微生物生物传感器来解决，但从特定工业废水中测量得到的传感器-BOD 值仍然低估了此类样品的 BOD 值。本研究旨在为乳制品废水中提供快速且更准确的 BOD 测量。与城市废水不同，乳制品工业废水含有许多大多数微生物不易利用的基质。因此，从乳制品废水中分离出的一种名为 Phyllosphaerae 的细菌被用于构建半特异性微生物生物传感器。以具有广泛基质谱但对乳制品废水无特异性的荧光假单胞菌为基础的通用微生物生物传感器被用作比较。用 OECD 合成废水对 BOD 生物传感器进行校准，并对不同的合成和实际废水样品进行了实验。结果表明，与基于荧光假单胞菌的生物传感器相比，半特异性的基于 Phyllosphaerae 的微生物生物传感器对含有乳衍生产品和乳清的废水更敏感。虽然基于 M. phyllosphaerae 的生物传感器对实际乳制品废水的 BOD7 值的低估程度为 25-32%，但这种细菌培养物比荧光假单胞菌更适合乳制品废水中的 BOD 监测，后者对相同的样品的低估程度为 46-61%。
