Environ Technol. 2014 Sep-Oct;35(17-20):2204-11. doi: 10.1080/09593330.2014.898700.
Microbial fuel cells (MFCs) have attracted considerable attention as potential biosensors. A MFC biosensor for rapid measurement of biochemical oxygen demand (BOD) has been recently studied. However, a standardized bacterial mixture inoculated in the MFC biosensor for BOD measurement is unavailable. Thus, the commercial application of a MFC biosensor is limited. In this study, a mediator-less MFC biosensor inoculated with known mixed cultures to quickly determine BOD concentration was tested. Optimal external resistance, operating temperature and measurement time for the MFC biosensor were determined to be 5000 omega, 35 degrees C and 12h, respectively. A good relationship between BOD concentration and voltage output, high reproducibility and long-term stability for the MFC biosensor was observed. The newly developed MFC biosensor was inoculated with a mixture of six bacterial strains (Thermincola carboxydiphila, Pseudomonas aeruginosa, Ochrobactrum intermedium, Shewanella frigidimarina, Citrobacter freundii and Clostridium acetobutylicum) capable of degrading complex organic compounds and surviving toxic conditions. The described MFC biosensor was able to successfully measure BOD concentrations below 240 mg L(-1) in real wastewater samples.
微生物燃料电池 (MFC) 作为潜在的生物传感器引起了相当大的关注。最近研究了一种用于快速测量生化需氧量 (BOD) 的 MFC 生物传感器。然而，用于 BOD 测量的 MFC 生物传感器中接种的标准化细菌混合物尚不可用。因此，MFC 生物传感器的商业应用受到限制。在这项研究中，测试了一种不使用介体的 MFC 生物传感器，该传感器接种了已知的混合培养物，可快速测定 BOD 浓度。确定 MFC 生物传感器的最佳外接电阻、工作温度和测量时间分别为 5000 欧姆、35 摄氏度和 12 小时。观察到 MFC 生物传感器的 BOD 浓度与电压输出之间存在良好的关系、高重现性和长期稳定性。新开发的 MFC 生物传感器接种了六种能够降解复杂有机化合物并在有毒条件下生存的细菌菌株（Thermincola carboxydiphila、Pseudomonas aeruginosa、Ochrobactrum intermedium、Shewanella frigidimarina、Citrobacter freundii 和 Clostridium acetobutylicum）。所描述的 MFC 生物传感器能够成功测量实际废水样品中低于 240mg/L 的 BOD 浓度。
