采用流式细胞免疫珠检测法检测成人前体 B 细胞急性淋巴细胞白血病患者外周血和骨髓样本中的 BCR-ABL 融合蛋白。

BCR-ABL fusion protein detection in peripheral blood and bone marrow samples of adult precursor B-cell acute lymphoblastic leukemia patients using the flow cytometric immunobead assay.

机构信息

Department of Immunology and Histocompatibility, Evangelismos General Hospital, Athens, Greece.

出版信息

Clin Chem Lab Med. 2012 Mar 3;50(9):1657-63. doi: 10.1515/cclm-2011-0686.

DOI:10.1515/cclm-2011-0686

PMID:22962228

Abstract

BACKGROUND

The ability to detect the BCR-ABL fusion gene in precursor B-cell acute lymphoblastic leukemia (pB-ALL) is essential for making accurate treatment decisions.

METHODS

We used a new flow cytometric immunobead assay for BCR-ABL fusion protein detection in peripheral blood and/or bone marrow samples from 38 adult pB-ALL patients and the results were compared with polymerase chain reaction (PCR) detection of BCR-ABL transcript.

RESULTS

The fusion protein was detected in peripheral blood and bone marrow samples from seven of the 38 (18%) patients, and results for both the p190 and p210 were confirmed by PCR. One case, which was positive by cytogenetics and fluorescence in situ hybridization (FISH), was negative by PCR but positive by flow cytometry. Another case, which was positive by PCR and negative by flow cytometry, was from a patient on steroid treatment.

CONCLUSIONS

The cytometric immunobead assay for BCR-ABL fusion protein detection was found to be suitable for the investigation of pB-ALL patients. This assay is reliable, rapid and simple to use for peripheral blood and bone marrow samples.

摘要

背景

检测前体 B 细胞急性淋巴细胞白血病（pB-ALL）中的 BCR-ABL 融合基因对于做出准确的治疗决策至关重要。

方法

我们使用一种新的流式细胞术免疫珠检测外周血和/或骨髓样本中的 BCR-ABL 融合蛋白，对 38 例成人 pB-ALL 患者进行检测，并将结果与 BCR-ABL 转录本的聚合酶链反应（PCR）检测进行比较。

结果

在 38 例患者中的 7 例（18%）外周血和骨髓样本中检测到融合蛋白，p190 和 p210 的结果均通过 PCR 得到确认。1 例经细胞遗传学和荧光原位杂交（FISH）证实为阳性的病例，PCR 结果为阴性但流式细胞术结果为阳性。另 1 例 PCR 结果为阳性而流式细胞术结果为阴性的病例来自正在接受类固醇治疗的患者。