Vulić Ana, Pleadin Jelka, Perši Nina, Mitak Mario
Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Zagreb, Croatia.
J Immunoassay Immunochem. 2012;33(4):369-76. doi: 10.1080/15321819.2012.655821.
The aim of this study was to determine feed and urinary levels of zearalenone. A total of 114 samples, 64 feeding stuffs (commodities, pig and cattle feed), and 50 urine samples were analyzed by the use of enzyme-linked immunosorbent assay (ELISA). Zearalenone was detected in 68.7% of feeding stuffs, while all urine samples except for four yearling samples were positive for zearalenone. The maximum zearalenone concentration in feeding stuffs and urine was 577 ng/g and 241.1 ng/mL, respectively. Although zearalenone concentrations in some samples were high, the risk for humans was negligible since the calculated concentrations in meat were below the tolerable daily intake (TDI).
本研究的目的是测定玉米赤霉烯酮在饲料和尿液中的含量。总共114个样本,包括64种饲料(商品饲料、猪饲料和牛饲料)以及50份尿液样本,采用酶联免疫吸附测定法(ELISA)进行分析。在68.7%的饲料样本中检测到了玉米赤霉烯酮,而除了4份一岁龄样本外,所有尿液样本中玉米赤霉烯酮均呈阳性。饲料和尿液中玉米赤霉烯酮的最高浓度分别为577纳克/克和241.1纳克/毫升。尽管一些样本中玉米赤霉烯酮的浓度较高,但由于计算得出的肉类中的浓度低于每日可耐受摄入量(TDI),因此对人类的风险可忽略不计。