Kuo Hsiao-Ting, Yeh Jay Z, Wu Po-Hua, Jiang Chii-Ming, Wu Ming-Chang
Department of Food Science, National Pingtung University of Science and Technology, Taiwan.
J Immunoassay Immunochem. 2012;33(4):377-87. doi: 10.1080/15321819.2012.655820.
This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG α antibody to superparamagnetic particle (SPIO-anti hCG α antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.
本研究旨在利用超顺磁性颗粒进行人绒毛膜促性腺激素（hCG）的酶联免疫吸附测定（SPIO-ELISA），以提高hCG的检测灵敏度。我们发现，N-(3-二甲氨基丙基)-N'-乙基碳二亚胺盐酸盐（EDC）是将抗hCGα抗体与超顺磁性颗粒（SPIO-抗hCGα抗体免疫磁珠）连接的最佳交联剂。为提高检测的特异性，采用辣根过氧化物酶（HRP）标记的抗hCGβ单克隆抗体，通过双抗体夹心ELISA法检测捕获的hCG。SPIO-ELISA用于测定hCG可将灵敏度提高到1 mIU/mL，这一灵敏度水平能够在妊娠早期诊断妊娠。
