Centro Regionale Antidoping A. Bertinaria, Orbassano (Turin), Italy.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(12):1893-900. doi: 10.1080/19440049.2012.719645. Epub 2012 Sep 11.
Prednisolone is a synthetic glucocorticoid widely employed in bovine clinical practice that may also be used illegally as a growth promoter. Recent in vitro and in vivo studies lend support to the hypothesis that prednisolone could be synthesised from cortisol in untreated cattle subjected to stressful events. To verify such a hypothesis, a field survey was conducted on urine samples collected from 131 guaranteed untreated cows and analysed for the presence of prednisolone and prednisone - in some instances also for cortisol and cortisone - with a validated LC/MS-MS method. None of the examined samples exhibited either prednisolone levels higher than the CCα limit (around 0.70 µg l⁻¹) or prednisone, being therefore officially compliant for both analytes. Trace amounts of prednisolone, approximately estimated in the range 0.1-0.3 µg l⁻¹ were found in only seven samples from cows also showing urinary cortisol and cortisone levels higher than those detected in negative specimens, as the result of a probable stress condition.
泼尼松龙是一种广泛应用于牛临床实践的合成糖皮质激素，也可能被非法用作生长促进剂。最近的体外和体内研究支持这样一种假设，即在经历应激事件的未经处理的牛中，泼尼松龙可能从皮质醇合成。为了验证这一假设，对从 131 头保证未经处理的奶牛采集的尿液样本进行了实地调查，并使用经过验证的 LC/MS-MS 方法分析了这些样本中是否存在泼尼松龙和泼尼松-在某些情况下还分析了皮质醇和可的松-。没有一个检查样本显示泼尼松龙水平高于 CCα 限量（约 0.70 µg l⁻¹）或泼尼松，因此这两种分析物均符合官方规定。仅在来自奶牛的七个样本中发现了痕量的泼尼松龙，其估计值约为 0.1-0.3 µg l⁻¹，这些奶牛的尿液皮质醇和可的松水平也高于阴性样本中检测到的水平，这是应激条件的结果。
