Postgraduate Program of Nutrition, Health Sciences Center, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Nutrition. 2012 Nov-Dec;28(11-12):1157-64. doi: 10.1016/j.nut.2012.04.011. Epub 2012 Sep 8.
Paraoxonase-2 (PON-2) is an intracellular antioxidant enzyme that can be modulated by polyphenols. The aim of this study was to verify whether yerba mate (Ilex paraguariensis), a plant species rich in phenolic compounds, modulates gene expression and the activity of PON-2 in macrophages in vitro and in monocytes from peripheral blood and monocyte-derived macrophages obtained after the ingestion of green or roasted yerba mate infusions by healthy subjects.
THP-1 macrophages were incubated with increasing amounts of yerba mate extracts or chlorogenic and caffeic acids (1-10 μmol/L). The in vivo effects of yerba mate or water (control) intakes were evaluated acutely (2 h after ingestion) and in the short term (after daily ingestion for 7 d) in 20 healthy women.
In general, there was no difference between the two kinds of yerba mate studied. Yerba mate extracts or chlorogenic acid at 1 and 3 μmol/L increased PON-2 relative gene expression in THP-1 macrophages (P < 0.05), whereas higher concentrations (5 and 10 μmol/L) increased the activity only. Caffeic acid induced PON-2 activity only. The acute ingestion of yerba mate infusions increased relative gene expression and PON-2 activity in monocytes (P < 0.05), whereas the consumption of yerba mate for 7 d increased PON-2 relative gene expression (P < 0.05) and had a tendency to increase PON-2 activity in monocytes and monocyte-derived macrophages.
It is suggested that green or roasted yerba mate modulates positively the mRNA relative expression and activity of the PON-2 enzyme in monocytes and macrophages, which may prevent cellular oxidative stress.
对氧磷酶 2（PON-2）是一种细胞内抗氧化酶，可被多酚类物质调节。本研究旨在验证富含多酚类化合物的巴拉圭冬青（Ilex paraguariensis）是否能调节体外巨噬细胞中 PON-2 的基因表达和活性，以及健康受试者饮用绿茶或烘烤的冬青叶浸提液后外周血单核细胞和单核细胞衍生的巨噬细胞中 PON-2 的活性。
用不同浓度的冬青叶提取物或绿原酸和咖啡酸（1-10 μmol/L）孵育 THP-1 巨噬细胞。在 20 名健康女性中，急性（摄入后 2 小时）和短期（每日摄入 7 天后）评估饮用冬青叶或水（对照）的体内作用。
一般来说，两种冬青叶没有差异。1 和 3 μmol/L 的冬青叶提取物或绿原酸增加了 THP-1 巨噬细胞中 PON-2 的相对基因表达（P < 0.05），而较高浓度（5 和 10 μmol/L）仅增加了活性。咖啡酸仅诱导 PON-2 活性。急性饮用冬青叶浸提液增加了单核细胞中 PON-2 的相对基因表达和活性（P < 0.05），而连续 7 天饮用冬青叶增加了单核细胞和单核细胞衍生的巨噬细胞中 PON-2 的相对基因表达（P < 0.05），并倾向于增加 PON-2 活性。
提示绿茶或烘烤的冬青叶可能通过调节单核细胞和巨噬细胞中 PON-2 酶的 mRNA 相对表达和活性来预防细胞氧化应激。
