Nabavi Seyed M, Nabavi Seyed F, Moghaddam Akbar H, Setzer William N, Mirzaei Morteza
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
An Acad Bras Cienc. 2012 Dec;84(4):1121-6. doi: 10.1590/s0001-37652012005000056. Epub 2012 Sep 6.
This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.
本研究旨在评估水飞蓟素对氟化钠诱导的大鼠心脏组织氧化应激的保护作用。在给予动物饮用含600 ppm氟化钠的水之前，先分别用20 mg/kg和10 mg/kg的水飞蓟素对其进行预处理。以10 mg/kg的维生素C作为标准抗氧化剂。在氟化钠中毒大鼠的组织匀浆中，硫代巴比妥酸反应性物质水平显著升高（59.36±2.19 nmol丙二醛当量/g组织），同时抗氧化酶活性降低（超氧化物歧化酶活性为64.27±1.98 U/g组织，过氧化氢酶活性为29.17±1.01 μmol/min/mg蛋白质），还原型谷胱甘肽水平降低（3.8±0.15 μg/mg蛋白质）。在给予动物氟化钠之前给予水飞蓟素可改变生化参数水平。
