Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, P.O.Box: 19945-546, Tehran, Iran.
Bull Environ Contam Toxicol. 2012 Mar;88(3):486-90. doi: 10.1007/s00128-011-0495-5. Epub 2011 Dec 6.
Curcumin is well known for its potent antioxidant activity. The result of numerous studies showed that antioxidants can protect against fluoride-induced toxicity. In the present study, protective effects of curcumin against sodium fluoride-induced toxicity in rat erythrocytes were evaluated. Curcumin (10 and 20 mg/kg) and vitamin C (10 mg/kg) were administrated intraperitoneally for 1 week followed by sodium fluoride (600 ppm) treatment for next week. Erythrocytes were isolated and superoxide dismutase and catalase activities as well as the levels of reduced glutathione, and lipid peroxidation were measured. The level of malondialdehyde in sodium fluoride treated rats (595.13 ± 20.23 nmol/g Hb) increased compared to the normal rats (315.44 ± 9.76 nmol/g Hb). Animals which were pretreated with curcumin at 20 mg/kg for 1 week prior to sodium fluoride intoxication showed significant reduction in the malondialdehyde level (320.22 ± 9.28 nmol/g Hb). Also, pretreated with curcumin (20 mg/kg) and vitamin C restored the superoxide dismutase and catalase activities and modified the level of reduced glutathione compared with control group (p > 0.01).
姜黄素具有很强的抗氧化活性。大量研究结果表明，抗氧化剂可以预防氟化物诱导的毒性。本研究评估了姜黄素对大鼠红细胞中氟化钠诱导的毒性的保护作用。姜黄素（10 和 20mg/kg）和维生素 C（10mg/kg）经腹腔注射给药 1 周，然后再用氟化钠（600ppm）处理 1 周。分离红细胞，测定超氧化物歧化酶和过氧化氢酶活性以及还原型谷胱甘肽水平和脂质过氧化水平。与正常大鼠（315.44 ± 9.76nmol/gHb）相比，氟化钠处理大鼠（595.13 ± 20.23nmol/gHb）的丙二醛水平升高。在氟化钠中毒前用姜黄素（20mg/kg）预处理 1 周的动物，丙二醛水平显著降低（320.22 ± 9.28nmol/gHb）。此外，与对照组相比，用姜黄素（20mg/kg）和维生素 C 预处理可恢复超氧化物歧化酶和过氧化氢酶活性，并改变还原型谷胱甘肽水平（p>0.01）。
