脐带血中的间充质干细胞群体是否随不同的妊娠时期而变化？
Does mesenchymal stem cell population in umbilical cord blood vary at different gestational periods?
机构信息
Department of Pediatrics, Division of Neonatology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
出版信息
Indian J Pediatr. 2013 May;80(5):375-9. doi: 10.1007/s12098-012-0844-3. Epub 2012 Sep 11.
OBJECTIVE
To identify and quantitatively determine Mesenchymal stem cells (MSCs) in the umbilical cord blood (UCB) of neonates born at different gestational periods.
METHODS
UCB was collected at birth in neonates of three different gestational groups. The mononuclear cells (MNCs) were phenotypically analyzed by flow cytometer.
RESULTS
The yield of total MNCs did not differ much with gestation; the average values were 22.6 ± 6.48 × 106 cells/ml. The MSCs were significantly higher in the lower gestation group. These were 0.0219 ± 0.012 %, 0.0044 ± 0.003 % and 0.0022 ± 0.003 % in 28 to 31 wk, 32 to 35 wk and >36 wk, respectively (P = 0.00). There was a significant inverse correlation between the gestational age and the presence of MSCs with a correlation co-efficient of -0.54 (P = 0.0001).
CONCLUSIONS
The MSCs population was significantly higher in infants born at lesser gestation than those born at term gestation.
目的
鉴定并定量检测不同胎龄新生儿脐带血中的间充质干细胞（MSCs）。
方法
在不同胎龄组的新生儿出生时采集脐血，用流式细胞仪对单个核细胞（MNC）进行表型分析。
结果
总 MNC 产量与胎龄差异不大，平均值为 22.6±6.48×106 个细胞/ml。MSCs 在较低胎龄组中明显更高，分别为 28 至 31 周、32 至 35 周和>36 周的 0.0219±0.012%、0.0044±0.003%和 0.0022±0.003%（P=0.00）。胎龄与 MSCs 存在呈显著负相关，相关系数为-0.54（P=0.0001）。
结论
与足月产婴儿相比，早产婴儿的 MSCs 群体明显更高。
Zotero 插件