Laboratorio Virologia, Dpto. SAMP, FCV, UNCPBA, Campus Universitario, Arroyo Seco S/N, Tandil, Argentina.
Arch Virol. 2013 Jan;158(1):63-70. doi: 10.1007/s00705-012-1459-8. Epub 2012 Sep 11.
Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2 0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.
感染牛白血病病毒（BLV）的牛根据其前病毒载量分为低和高前病毒载量谱（分别为 LPL 和 HPL）。这些动物的血液被用于感染绵羊，以获得多个整合前病毒的相同副本。从所有感染的绵羊中扩增并测序了 BLV 的 env 片段。获得的序列与已发表的 BLV 基因组序列进行比较，产生了三个聚类。这些突变不能归因于前病毒从牛传播到绵羊，以及随后的扩增和测序。两个不同的前病毒载量谱的描述、BoLA-DRB3.2 0902 等位基因与 LPL 谱的关联、完整 BLV 序列的可用性，以及对经过精心特征描述的牛的 env 基因的一个可变区的比较，仍然不足以解释为什么每个牛群中都有对 BLV 传播具有抗性的动物存在。
