Biosciences Department, COMSATS University, Islamabad, Pakistan.
National Veterinary Laboratory, Park Road, Islamabad, Pakistan.
Sci Rep. 2022 Mar 9;12(1):4161. doi: 10.1038/s41598-022-08181-5.
Worldwide, breast cancer has an eminent morbidity and mortality rate, as it is a neoplastic disease among females. The query of the prospective danger of bovine leukemia virus (BLV) to humans is an old but exceedingly topical focus of scientific debate. The objective of the current study was to determine the possible relationship between BLV and breast cancer. A total of 2710 formalin-fixed paraffin-embedded (FFPE) breast cancer samples were selected regardless of the age, ethnicity, or municipality origin of the subjects. The presence of BLV in human breast cancer was determined through nested PCR by amplifying tax and gag genes followed by partial sequencing. Homology was confirmed by using the online BLAST Tool. BLV genes were found to be positive in 26.8% (728/2710) of the samples from breast cancer patients and 10% (10/80) of the samples without cancer (negative control). The results indicated a correlation between the presence of the BLV gene and breast cancer (odds ratio = 0.3889; confidence interval = 1,18; p = 0.0029). The current findings suggest a possible link between BLV and human breast carcinoma. Therefore, screening cattle herds and milk products is suggested to reduce the viral transmission risk to humans.
在全球范围内，乳腺癌的发病率和死亡率都很高，因为它是女性中的一种肿瘤疾病。牛白血病病毒（BLV）对人类潜在危险的问题是一个古老但非常热门的科学争论焦点。本研究的目的是确定 BLV 与乳腺癌之间可能存在的关系。选择了总共 2710 例福尔马林固定石蜡包埋（FFPE）乳腺癌样本，无论研究对象的年龄、种族或所在城市如何。通过巢式 PCR 扩增 tax 和 gag 基因并进行部分测序来确定人类乳腺癌中 BLV 的存在。通过在线 BLAST 工具确认同源性。在 2710 例乳腺癌患者样本中，有 26.8%（728/2710）的样本和 10%（10/80）的无癌症（阴性对照）样本中发现 BLV 基因呈阳性。结果表明 BLV 基因的存在与乳腺癌之间存在相关性（比值比=0.3889；置信区间=1,18；p=0.0029）。目前的研究结果表明 BLV 与人类乳腺癌之间可能存在联系。因此，建议对牛群和奶制品进行筛查，以降低病毒向人类传播的风险。
