Kanje M, Walum E, Edstróm A
Z Mikrosk Anat Forsch. 1979;93(3):487-96.
Cultured human glioma cells (138 MG) exposed to dibutyryl cyclic AMP (dbc-AMP; 0.1--5 mM) attained an arborized shape with thin processes extending from a rounded cell body. Cytochalasin B (CB; 1--1 muM) induced similar morphological changes. The processes in both dbc-AMP and CB treated cells were formed by retraction of the cell margin. Colchicine (1muM) completely and liver treated phalloidin (0.1 mg/ml) partially inhibited the morphological alterations induced by dbc-AMP and CB. Dbc-AMP was found to arrest cell movement, cell division and uptake of 2-deoxy-D-glucose. CB has the same effects but was more potent. The effects of dbc-AMP and CB could be due to interference with a common cellular structure, e.g. microfilaments.
暴露于二丁酰环磷腺苷（dbc-AMP；0.1-5 mM）的培养人胶质瘤细胞（138 MG）呈现出树状形态，从圆形细胞体伸出细长的突起。细胞松弛素B（CB；1-1 μM）诱导了类似的形态变化。dbc-AMP处理细胞和CB处理细胞中的突起都是由细胞边缘收缩形成的。秋水仙碱（1 μM）完全抑制，而荧光鬼笔环肽（0.1 mg/ml）部分抑制了dbc-AMP和CB诱导的形态改变。发现dbc-AMP可阻止细胞移动、细胞分裂以及2-脱氧-D-葡萄糖的摄取。CB有相同作用，但效力更强。dbc-AMP和CB的作用可能是由于干扰了一种共同的细胞结构，如微丝。
