Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Chin J Integr Med. 2012 Oct;18(10):782-7. doi: 10.1007/s11655-012-1178-8. Epub 2012 Sep 11.
To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography (HPLC) and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae.
The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J'sphere ODS-H80 column (4.6 mm×250 mm, 5 μm) with acetonitrile-water-tetrahydrofuran-acetic acid (41:59:1:0.2, v/v/v/v) as the mobile phase. The detection wavelength was 225 nm, the column temperature was 35°C, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a Kromasil-C₁₈ column (4.6 mm×250 mm, 4 μm) and a gradient elution of acetonitrile (A) and 0.05% aqueous phosphoric acid (B) as the mobile phase. The detection wavelength was 237 nm, the column temperature was 35 °C, the flow rate was 1.0 mL/min, and the injection volume was 10 μL.
The calibration curves of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin showed good linear relationships (r>0.99). The recoveries of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin were 96.59%, 104.18%, 101.91%, 97.75%, and 97.95%, respectively. The concentrations of the components in processed Fructus Evodiae were obviously different to those in unprocessed Fructus Evodiae.
The developed method is rapid and accurate. The results provide a reference for processed Fructus Evodiae and the changes that could be expected in its effects compared to unprocessed Fructus Evodiae.
采用高效液相色谱法（HPLC）评价吴茱萸药材与甘草提取液共炮制前后 5 种成分（吴茱萸碱、甘草酸）的含量变化，为吴茱萸生品及炮制品的不同临床应用提供科学依据。
采用 YMC J'sphere ODS-H80 色谱柱（4.6mm×250mm，5μm），以乙腈-水-四氢呋喃-冰醋酸（41:59:1:0.2，v/v/v/v）为流动相，检测波长 225nm，柱温 35℃，流速 1.0mL/min，进样量 10μL，测定吴茱萸生品及炮制品中 5 种成分的含量；采用 Kromasil-C₁₈ 色谱柱（4.6mm×250mm，4μm），梯度洗脱乙腈（A）-0.05%磷酸水溶液（B）为流动相，检测波长 237nm，柱温 35℃，流速 1.0mL/min，进样量 10μL，测定甘草药材中 5 种成分的含量。
吴茱萸内酯、吴茱萸碱、吴茱萸次碱、甘草苷、甘草酸的线性关系良好（r>0.99）。吴茱萸内酯、吴茱萸碱、吴茱萸次碱、甘草苷、甘草酸的平均回收率分别为 96.59%、104.18%、101.91%、97.75%、97.95%。炮制前后吴茱萸中 5 种成分的含量明显不同。
该方法快速、准确，为吴茱萸炮制品的研究和其与生品相比可能发生的药效变化提供了参考。
