[采用QAMS法测定吴茱萸中吴茱萸碱、吴茱萸次碱和吴茱萸新碱的含量]
[Assay of evodin, evodiamine and rutaecarpine in Fructus Evodiae by QAMS].
作者信息
Song Yafang, Wang Zhimin, Zhu Jingjing, Yan Lihua, Zhang Qiwei, Gong Muxin, Gao Huimin, Feng Weihong, Wang Weihao
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2781-5.
OBJECTIVE
To develop a Quantitative Assay of Multi-components by Single - marker (QAMS) for simultaneous determination of three components in Fructus Evodiae, and examine the feasibility of using the relative correction factors between the different types of compounds.
METHOD
Rutaecarpine was selected as the internal reference substance; the relative correction factors of evodin and evodiamine were calculated. The contents of three components in 11 batches of samples were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of their quantitative results.
RESULT
No obvious differences (RSD < 5%) were found in the quantitative results of evodin and evodiamine in 11 batches of Fructus Evodiae determined by the two methods.
CONCLUSION
It is feasible and suitable to determine evodin and evodiamine in Fructus Evodiae by QAMS, and this method can be used for a certain different types of compounds.
目的
建立一种单指标多成分定量法（QAMS）同时测定吴茱萸中3种成分，并考察不同类型化合物间相对校正因子使用的可行性。
方法
选取吴茱萸次碱作为内参物，计算吴茱萸碱和吴茱萸次碱的相对校正因子。采用外标法和QAMS法分别测定11批样品中3种成分的含量，通过比较两种方法的定量结果评价QAMS法的有效性。
结果
两种方法测定11批吴茱萸中吴茱萸碱和吴茱萸次碱的定量结果无明显差异（RSD＜5%）。
结论
采用QAMS法测定吴茱萸中吴茱萸碱和吴茱萸次碱可行且适用，该方法可用于一定范围内不同类型的化合物。
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