Park Kun, Lee Ji-Hye, Cho Ho-Chan, Cho Sun-Young, Cho Jae-We
Department of Dermatology, College of Medicine Eulji University, Seoul.
Oncol Lett. 2010 Mar;1(2):289-292. doi: 10.3892/ol_00000051. Epub 2010 Mar 1.
There is considerable evidence that glucosamine exerts an inhibitory effect on inflammatory cytokine expression in cells. Glucosamine has been recommended as a promising anti-inflammatory modulator, which has been applied in clinical trials for attenuation of the inflammatory process. However, it is unknown whether glucosamine reduces the expression of TNF-α-induced inflammatory cytokines in HaCaT cells. The anti-inflammatory effects of curcumin in HaCaT cells have been extensively investigated in several studies. Thus, in this study we investigated the expression of IL-6, IL-8, TNF-α and IL-1β in glucosamine-treated HaCaT cells, and the effects of glucosamine were compared to those of curcumin-treated HaCaT cells. Our data showed that the expression of IL-6, IL-8, TNF-α and IL-1β was decreased by glucosamine treatment in the HaCaT cells. In contrast, the expression of IL-6, IL-8, TNF-α and IL-1β was not attenuated by glucosamine treatment in the TNF-α-treated HaCaT cells. Notably, curcumin induced an increased expression of IL-8 and IL-1β in the HaCaT cells, but not that of IL-6 and TNF-α. On the other hand, curcumin attenuated the expression of IL-6 and IL-8 in the TNF-α-treated HaCaT cells. Our data indicated that glucosamine induced the down-regulation of IL-6, IL-8, TNF-α and IL-1β expression in the HaCaT cells. However, the stimulation of TNF-α abolished the inhibitory effects of glucosamine on the expression of inflammatory cytokines in the HaCaT cells. Thus, even though glucosamine induces the down-regulation of inflammatory cytokines in HaCaT cells, the anti-inflammatory role of glucosamine in TNF-α-mediated inflammatory skin diseases should be investigated.
有大量证据表明，氨基葡萄糖对细胞中炎性细胞因子的表达具有抑制作用。氨基葡萄糖已被推荐为一种有前景的抗炎调节剂，并已应用于减轻炎症过程的临床试验中。然而，尚不清楚氨基葡萄糖是否能降低肿瘤坏死因子-α（TNF-α）诱导的人永生化角质形成细胞（HaCaT细胞）中炎性细胞因子的表达。姜黄素在HaCaT细胞中的抗炎作用已在多项研究中得到广泛研究。因此，在本研究中，我们研究了氨基葡萄糖处理的HaCaT细胞中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、TNF-α和白细胞介素-1β（IL-1β）的表达，并将氨基葡萄糖的作用与姜黄素处理的HaCaT细胞的作用进行了比较。我们的数据表明，氨基葡萄糖处理可降低HaCaT细胞中IL-6、IL-8、TNF-α和IL-1β的表达。相反，在TNF-α处理的HaCaT细胞中，氨基葡萄糖处理并未减弱IL-6、IL-8、TNF-α和IL-1β的表达。值得注意的是，姜黄素可诱导HaCaT细胞中IL-8和IL-1β的表达增加，但不会诱导IL-6和TNF-α的表达增加。另一方面，姜黄素可减弱TNF-α处理的HaCaT细胞中IL-6和IL-8的表达。我们的数据表明，氨基葡萄糖可诱导HaCaT细胞中IL-6、IL-8、TNF-α和IL-1β表达的下调。然而，TNF-α的刺激消除了氨基葡萄糖对HaCaT细胞中炎性细胞因子表达的抑制作用。因此，尽管氨基葡萄糖可诱导HaCaT细胞中炎性细胞因子的下调，但氨基葡萄糖在TNF-α介导的炎性皮肤病中的抗炎作用仍有待研究。
