Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.
Org Lett. 2012 Sep 21;14(18):4782-5. doi: 10.1021/ol302071f. Epub 2012 Sep 11.
We describe a general method for the elongation of nucleoside oligophosphate chains by means of cyanoethyl (CE) phosphorimidazolides. Though the method requires a phosphorylation and subsequent deprotection reaction, both steps could be achieved in one pot without isolation/purification of the initial phosphorylation product. We have also found that pyrophosphate bond formation by this method is significantly accelerated by microwave irradiation.
我们描述了一种通过氰乙基（CE）亚磷酰胺咪唑酯延长寡核苷酸链的通用方法。虽然该方法需要进行磷酸化和随后的脱保护反应，但这两个步骤可以在不分离/纯化初始磷酸化产物的情况下在一个锅中完成。我们还发现，通过这种方法形成焦磷酸键的速度可以通过微波辐射显著加快。
