Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Langmuir. 2012 Oct 2;28(39):13788-92. doi: 10.1021/la302902m. Epub 2012 Sep 17.
Polyhistidine peptide dendrimer self-assembles on CdSe/ZnS quantum dots (QDs) with very high affinity and stability, a property ascribable to its multivalent geometry. Here we designed a fluorescent protein, GCN-mCherry, that exists as an oligomeric bundled structure in solution as well as on the surface to imitate the structure of a synthetic dendrimer. GCN-mCherry forms a very stable assembly with QDs, which can resist displacement by 500 mM imidazole and the dendrimer peptide, as measured by the Förster resonance energy transfer from QD to mCherry. Our work manifested a prominent stability enhancement of protein-nanoparticle assembly through directional ligand-ligand interaction on the surface.
多聚组氨酸肽树枝状大分子与 CdSe/ZnS 量子点（QDs）通过非常高的亲和力和稳定性自组装，这种特性归因于其多价几何形状。在这里，我们设计了一种荧光蛋白 GCN-mCherry，它在溶液中以及表面上都以多聚体捆绑结构的形式存在，以模拟合成树枝状大分子的结构。GCN-mCherry 与 QDs 形成非常稳定的组装体，可抵抗 500mM 咪唑和树枝状大分子肽的置换，这可以通过 QD 到 mCherry 的Förster 共振能量转移来测量。我们的工作通过表面上的定向配体-配体相互作用，显著增强了蛋白质-纳米颗粒组装体的稳定性。
