Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Hereditas. 2012 Aug;149(4):146-52. doi: 10.1111/j.1601-5223.2012.02261.x. Epub 2012 Aug 27.
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious yield-limiting factor for wheat production worldwide. The objective of this study was to identify and map a stripe rust resistance gene in wheat line Shaannong 104 using SSR markers. F(1), F(2) and F(3) populations from Shaannong 104/Mingxian 169 were inoculated with Chinese Pst race CYR32 in a greenhouse. Shaannong 104 carried a single dominant gene, YrSN104. Six potential polymorphic SSR markers identified in bulk segregant analysis were used to genotype F(2) and F(3) families. YrSN104 was closely linked with all six SSR markers on chromosome 1BS with genetic distances of 2.0 cM (Xgwm18, Xgwm273, Xbarc187), 2.6 cM (Xgwm11, Xbarc137) and 5.9 cM (Xbarc240). Pedigree analysis, pathogenicity tests using 26 Pst races, haplotyping of associated markers on isogenic lines carrying known stripe rust resistance genes, and associations with markers suggested that YrSN104 was a new resistance gene or an allele at the Yr24/Yr26 locus on chromosome 1BS. Deployment of YrSN104 singly or in combination to elite genotypes could play an effective role to lessen yield losses caused by stripe rust.
条锈病由条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici，Pst）引起，是全球范围内限制小麦产量的严重因素。本研究旨在利用 SSR 标记，鉴定和定位小麦品种山农 104 中的条锈病抗性基因。使用中国 Pst 生理小种 CYR32 对山农 104/Mingxian 169 的 F1、F2 和 F3 群体进行温室接种。山农 104 携带一个显性单基因 YrSN104。在 bulk segregant analysis 中鉴定的六个潜在多态性 SSR 标记被用于对 F2 和 F3 家系进行基因分型。YrSN104 与 1BS 染色体上的六个 SSR 标记紧密连锁，遗传距离分别为 2.0 cM（Xgwm18、Xgwm273、Xbarc187）、2.6 cM（Xgwm11、Xbarc137）和 5.9 cM（Xbarc240）。系谱分析、使用 26 个 Pst 生理小种进行的致病性测试、携带已知条锈病抗性基因的同型系相关标记的单倍型分析以及与标记的关联表明，YrSN104 是一个新的抗性基因或 1BS 染色体上 Yr24/Yr26 位点的等位基因。单独或组合使用 YrSN104 可以有效减少条锈病引起的产量损失。
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