双光子荧光探针用于镁/钙离子活性的双色成像。

Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes.

机构信息

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

出版信息

Anal Chem. 2012 Oct 2;84(19):8110-3. doi: 10.1021/ac302210v. Epub 2012 Sep 19.

DOI:10.1021/ac302210v

PMID:22967146

Abstract

We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg(2+) (Mg(2+)) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca(2+) (Ca(2+)), FMg2 allows dual-color imaging of Mg(2+)/Ca(2+) activities in live cells and Mg(2+) /Ca(2+) distributions in live tissues at a depth of 100-200 μm.

摘要

我们报告了两种可通过双光子显微镜选择性检测活细胞和组织内游离镁离子(Mg2+)的双光子探针（FMg1 和 FMg2）。与用于近膜钙离子(Ca2+)的双光子探针 BCaM 结合，FMg2 可在 100-200μm 的深度对活细胞内的 Mg2+/Ca2+活性以及活组织内的Mg2+/Ca2+分布进行双色成像。