Division of Energy Systems Research, Ajou University, Suwon, 443-749 Korea.
Chem Asian J. 2011 Jan 3;6(1):58-69. doi: 10.1002/asia.201000542.
Two-photon microscopy (TPM) has become an indispensible tool in biology and medicine owing to the capability of imaging the intact tissue for a long period of time. To make it a versatile tool in biology, a variety of two-photon probes for specific applications are needed. In this context, many research groups are developing two-photon probes for various applications. In this Focus Review, we summarize recent results on model studies and selected examples of two-photon probes that can detect intracellular free metal ions in live cells and tissues to provide a guideline for the design of useful two-photon probes for various in vivo imaging applications.
双光子显微镜(TPM)由于能够长时间对完整组织进行成像,已成为生物学和医学中不可或缺的工具。为了使它成为生物学中的通用工具,需要针对特定应用开发各种双光子探针。在这种情况下,许多研究小组正在开发用于各种应用的双光子探针。在本综述中,我们总结了有关模型研究和选定的双光子探针示例的最新结果,这些探针可以检测活细胞和组织中的细胞内游离金属离子,为设计用于各种体内成像应用的有用双光子探针提供了指导。