Sprygin A V, Nikonova Z B, Zinyakov N G
Laboratory of Livestock Disease Diagnostics, Federal Centre for Animal Health, Vladimir, 600901, Russia.
Avian Pathol. 2012 Oct;41(5):459-63. doi: 10.1080/03079457.2012.711464. Epub 2012 Sep 11.
A total of 79 liver samples from clinically sick and asymptomatic chickens were tested for avian hepatitis E virus (aHEV). Samples were received from 19 farms, five of which tested positive with primers targeting the ORF2 capsid gene. The phylogenetic analysis of a 242-base-pair fragment demonstrated that the Russian aHEV isolates share between 78.2 and 96.2% over the fragment sequenced, whereas the nucleotide sequence identities between the Russian isolates and the other representatives from GeneBank varied from 76.3 to 96.2%. The homology between the studied hepatitis E viruses and swine hepatitis E virus varied between 46.9 to 48.1%. The most divergent isolate aHEV16050 showed homology of 82.6% as compared with the strains in the dendrogram. The three positive hepatitis E virus samples (aHEV16279, aHEV16050 and aHEV18196) did not cluster with the European genotype 3 as expected due to the close location of Russia to Europe, nor did they with the other two genotypes, separating to a distinct branch. The aHEV16211 grouped together with European and Chinese isolates, and the aHEV18198 with Canadian ones.
对来自临床患病和无症状鸡的79份肝脏样本进行了禽戊型肝炎病毒（aHEV）检测。样本来自19个农场，其中5个农场使用靶向ORF2衣壳基因的引物检测呈阳性。对一个242个碱基对片段的系统发育分析表明，俄罗斯aHEV分离株在测序片段上的相似度为78.2%至96.2%，而俄罗斯分离株与基因库中其他代表株之间的核苷酸序列同一性在76.3%至96.2%之间。所研究的戊型肝炎病毒与猪戊型肝炎病毒之间的同源性在46.9%至48.1%之间。与系统发育树中的毒株相比，差异最大的分离株aHEV16050显示出82.6%的同源性。由于俄罗斯与欧洲地理位置接近，三个戊型肝炎病毒阳性样本（aHEV16279、aHEV16050和aHEV18196）并未如预期那样与欧洲基因型3聚类，也未与其他两种基因型聚类，而是分离到一个独特的分支。aHEV16211与欧洲和中国的分离株聚在一起，aHEV18198与加拿大的分离株聚在一起。
