[湖北省武汉市啮齿动物宿主中汉坦病毒S片段的基因分析]

[Genetic analysis on S segment of hantaviruses in rodent hosts from Wuhan area, Hubei province].

作者信息

Liu Dong-ying, Liu Jing, Li Jin-lin, Chen Wen, Luo Fan, Li Qing, Yang Zhan-qiu

机构信息

State Key Laboratory of Virology, Institute of Medical Virology, Wuhan University, Wuhan 430071, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2012 Aug;33(8):828-31.

PMID:22967338

Abstract

OBJECTIVE

To investigate the infection and genotype of hantaviruses in rodents from Wuhan area, Hubei province.

METHODS

Rodents were trapped in fields and residential areas of Xinzhou and Jiangxia districts of Wuhan in autumn and winter seasons, from 2000 to 2003 and from 2009 to 2011. Trapped rodents were identified, and hantavirus antigens were detected in the lung tissues with indirect immunofluorescence assay (IFA). Partial S segment sequences were amplified with RT-PCR in hantavirus antigen positive samples and then sequenced. Phylogenetic tree was constructed to analyze the genetic characteristics of hantaviruses.

RESULTS

From 2000 to 2003, 437 rodents were trapped, with 24 (5.49%) lung tissues showed hantavirus antigen positive. From 2009 to 2011, 173 rodents were trapped and 7 (4.05%) were hantavirus antigen positive. Rattus norvegicus were the dominant species of rodents. Partial S segment sequences were amplified from 22 samples with Hantaan and Seoul viruses specific primers and sequenced. Partial S segments of Seoul viruses (nucleotide 588 - 1147) were amplified from 17 rodents (13 R. norvegicus and 4 Apodemus agrarius). Seven of these sequences belonged to 3 genetic lineage, while two novel genetic lineages were formed by 9 and 1 sequences, respectively. Partial S segments of Hantaan viruses (nucleotide 615 - 1141) were amplified from 5 A. agrarius. One of these sequences belonged to 7 genetic lineages, and 4 sequences formed one novel genetic subtype.

CONCLUSION

Hantaan and Seoul viruses co-circulated in Wuhan area, Hubei province. Novel genetic lineages were identified in this study and Seoul virus might have caused spillover infection in A. agrarius.

摘要

目的

调查湖北省武汉市地区啮齿动物中汉坦病毒的感染情况及基因型。

方法

于2000年至2003年以及2009年至2011年的秋冬季节，在武汉市新洲区和江夏区的田野及居民区捕获啮齿动物。对捕获的啮齿动物进行鉴定，采用间接免疫荧光法（IFA）检测肺组织中的汉坦病毒抗原。对汉坦病毒抗原阳性样本用逆转录聚合酶链反应（RT-PCR）扩增部分S片段序列，然后进行测序。构建系统发育树分析汉坦病毒的遗传特征。

结果

2000年至2003年，共捕获437只啮齿动物，其中24只（5.49%）肺组织汉坦病毒抗原呈阳性。2009年至2011年，捕获173只啮齿动物，7只（4.05%）汉坦病毒抗原呈阳性。褐家鼠是啮齿动物的优势种。用汉滩病毒和汉城病毒特异性引物从22个样本中扩增出部分S片段序列并测序。从17只啮齿动物（13只褐家鼠和4只黑线姬鼠）中扩增出汉城病毒的部分S片段（核苷酸588 - 1147）。其中7个序列属于3个遗传谱系，另外9个和1个序列分别形成了2个新的遗传谱系。从5只黑线姬鼠中扩增出汉滩病毒的部分S片段（核苷酸615 - 1141）。其中1个序列属于7个遗传谱系，4个序列形成了1个新的遗传亚型。