Zhang Yong-zhen, Xiao Qi-you, Li Ming-hui, Zou Yang, Lv Wei, Dai De-fang, Chen Hua-xin
National Institute of Communicable Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Jan;28(1):65-9.
To explore the hantavirus infection and their genotype in rodents in Hunan.
Hantavirus antigens in the rat lungs from Hunan province were detected by immunofluorescence assay. Partial S and M segment in antigen-positive samples were amplified by RT-PCR, and then sequenced. The phyologenetic trees were constructed for the analysis of genetic characters of hantavirus.
A total of 344 rats were trapped in the main epidemic area of Hunan province, and hantavirus antigens were found in 6 of the 344 rats( 1.74% ).The phylogenetic trees constructed by partial S segment( nt 620-990) or partial G2 segment (nt 2001- 2301) showed that the hantaviruses carried by Rattus norvegicus, R . flabipectus and R. rattoides from Xiangxiang district were genetic subtype SEOV4. The virus carried by R. norvegicus in Ningyuan district was phylogenetically different from the known SEOV. The hantavirus carried by Mus musculus from Shimen district was genetic subtype HTNV4.
The hantaviruses in the main epidemic areas in Hunan province mainly belonged to SEOV, and R. flabipectus and R. rattoides carried the same genotype of SEOV as R. norvegicus.
探讨湖南省啮齿动物中汉坦病毒感染情况及其基因型。
采用免疫荧光法检测湖南省大鼠肺组织中的汉坦病毒抗原。对抗原阳性样本的部分S和M片段进行逆转录聚合酶链反应（RT-PCR）扩增并测序，构建系统发育树分析汉坦病毒的遗传特征。
在湖南省主要疫区共捕获344只大鼠，其中6只（1.74%）检测到汉坦病毒抗原。根据部分S片段（核苷酸620 - 990）或部分G2片段（核苷酸2001 - 2301）构建的系统发育树显示，来自湘乡地区的褐家鼠、黄毛鼠和社鼠携带的汉坦病毒为基因亚型SEOV4。宁远地区的褐家鼠携带的病毒在系统发育上与已知的SEOV不同。石门地区的小家鼠携带的汉坦病毒为基因亚型HTNV4。
湖南省主要疫区的汉坦病毒主要属于SEOV，黄毛鼠和社鼠携带与褐家鼠相同基因型的SEOV。
