Chen Min, Yao Shi-tang, Ma Yan-ling, He Xiang, Wang Ji-bao, Cheng Chun-lin, Yang Jin, Su Ying-zhen, Li Yan-ling, Duan Song, Chen Hui-chao, Fu Li-Ru, Jia Man-hong, Lu Lin
Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2012 Sep;33(9):883-7.
To investigate the distribution of HIV-1 subtypes in Dehong prefecture, Yunnan province, in 2011.
300 HIV-1 positive plasma samples were collected from Jan. 2011 to May 2011 in Dehong prefecture. HIV-1 gag genes and env genes were amplified by nested-polymerase chain reaction (PCR) from viral RNA. After sequencing, the HIV-1 subtypes were determined by phylogenetic analysis.
Based on the phylogenetic trees of gag gene and env gene fragments, a total of 222 samples were genotyped. Subtype C was the predominant strain in Dehong (43.2%, 96/222), followed by unique recombinant forms (URFs, 27.0%, 60/222), CRF01_AE (21.2%, 47/222), CRF08_BC (5.0%, 11/222), B' (2.3%, 5/222) and CRF07_BC (1.4%, 3/222). Subtype C strains were predominant in both heterosexually transmitted population and intravenous drug users (IDUs), but different subtype distribution patterns were found in these two populations. All 6 genotypes including subtype C (40.7%, 70/172), CRF01_AE (25.0%, 43/172), and URFs (25.0%, 43/172) found in this area among heterosexually transmitted population, which showed the diversity of genotypes in this population. Except subtype B' and CRF07_BC, the other 3 subtypes and URFs were detected among IDUs, mainly including subtype C (54.8%, 23/42) and URFs (38.1%, 16/42), which showed the concentration trend of genotypes distribution among IDUs. The proportion of URFs increased significantly in this area, including the new BC recombinants (41.7%, 25/60) and CRF01_AE relative URFs (58.3%, 35/60). However, the distributions of these two URFs among heterosexually transmitted population and IDUs showed no statistical significance.
The distribution of HIV-1 strains prevailing in Dehong prefecture was diversity, including 5 subtypes and a variety of URFs, of which subtype C was the predominant strain. The distribution patterns of subtype were different among different populations.
调查2011年云南省德宏州HIV-1亚型的分布情况。
于2011年1月至2011年5月在德宏州采集300份HIV-1阳性血浆样本。从病毒RNA中通过巢式聚合酶链反应（PCR）扩增HIV-1 gag基因和env基因。测序后，通过系统发育分析确定HIV-1亚型。
基于gag基因和env基因片段的系统发育树，共对222份样本进行了基因分型。C亚型是德宏州的主要毒株（43.2%，96/222），其次是独特重组型（URFs，27.0%，60/222）、CRF01_AE（21.2%，47/222）、CRF08_BC（5.0%，11/222）、B'（2.3%，5/222）和CRF07_BC（1.4%，3/222）。C亚型毒株在异性传播人群和静脉吸毒者（IDUs）中均占主导地位，但在这两个人群中发现了不同的亚型分布模式。在该地区异性传播人群中发现的所有6种基因型包括C亚型（40.7%，70/172）、CRF01_AE（25.0%，43/172）和URFs（25.0%，43/172），这表明该人群中基因型具有多样性。除B'亚型和CRF07_BC外，在IDUs中检测到其他3种亚型和URFs，主要包括C亚型（54.8%，23/42）和URFs（38.1%，16/42），这表明IDUs中基因型分布呈集中趋势。该地区URFs的比例显著增加，包括新的BC重组型（41.7%，25/60）和CRF01_AE相关的URFs（58.3%，35/60）。然而，这两种URFs在异性传播人群和IDUs中的分布无统计学差异。
德宏州流行的HIV-1毒株分布具有多样性，包括5种亚型和多种URFs，其中C亚型是主要毒株。不同人群中亚型的分布模式不同。
