Yang Ji-long, Cogdell David, Eddy James, Trent Jonathan, Price Nathan, Zhang Wei
Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Hospital, China.
Zhonghua Zhong Liu Za Zhi. 2012 Jul;34(7):497-500. doi: 10.3760/cma.j.issn.0253-3766.2012.07.005.
Our previous study shows that PURNE2 mRNA plays an important role in the differential diagnosis of leiomyosarcoma and gastrointestinal stromal tumor (GIST). Non-coding RNA PCA3 locates in the intron of PRUNE2 and may play a role in PRUNE2 expression. The aim of this study was to explore the expression of PCA3 mRNA and PRUNE2 in leiomyosarcoma and their correlation.
The expression of PRUNE2 mRNA was analyzed by agilent gene expression microarray CHIP in 31 leiomyosarcomas and 37 GISTs, and the correlation of the PRUNE2 expression and prognosis of leiomyosarcoma was predicted. Real-Time PCR assay was used to detect the mRNA levels of PCA3 and PRUNE2 in 13 leiomyosarcomas and to investigate their correlation. Seven prostate cancer tissues were used as control of PCA3.
The level of PRUNE2 mRNA expression was significantly higher in the 31 leiomyosarcomas than that in the 37 GISTs, and the level of PRUNE2 mRNA expression was correlated with survival of the leiomyosarcoma patients. Compared with prostate cancer, the non-coding RNA PCA3 expression level was significantly lower in leiomyosarcoma, and it had no correlation with the prognosis of leiomyosarcoma. Most importantly, the PRUNE2 and PCA3 mRNA expressions were both upregulated in leiomyosarcoma and showed a significant positive correlation.
Our findings demonstrate for the first time that PRUNE2 expression is correlated with the survival of leiomyosarcoma patients. Furthermore, non-coding RNA PCA3, which locates in the intron of PRUNE2, has a significant positive correlation with PRUNE2 and may play an important role in the pathogenesis of leiomyosarcoma.
我们之前的研究表明，PRUNE2 mRNA在平滑肌肉瘤与胃肠道间质瘤（GIST）的鉴别诊断中发挥重要作用。非编码RNA PCA3定位于PRUNE2的内含子中，可能在PRUNE2表达中发挥作用。本研究旨在探讨PCA3 mRNA和PRUNE2在平滑肌肉瘤中的表达及其相关性。
采用安捷伦基因表达微阵列芯片分析31例平滑肌肉瘤和37例GIST中PRUNE2 mRNA的表达，并预测PRUNE2表达与平滑肌肉瘤预后的相关性。采用实时荧光定量PCR检测13例平滑肌肉瘤中PCA3和PRUNE2的mRNA水平，并研究它们的相关性。选取7例前列腺癌组织作为PCA3的对照。
31例平滑肌肉瘤中PRUNE2 mRNA表达水平显著高于37例GIST，且PRUNE2 mRNA表达水平与平滑肌肉瘤患者的生存率相关。与前列腺癌相比，平滑肌肉瘤中非编码RNA PCA3的表达水平显著降低，且与平滑肌肉瘤的预后无关。最重要的是，平滑肌肉瘤中PRUNE2和PCA3的mRNA表达均上调，且呈显著正相关。
我们的研究首次表明，PRUNE2表达与平滑肌肉瘤患者的生存率相关。此外，定位于PRUNE2内含子的非编码RNA PCA3与PRUNE2具有显著正相关，可能在平滑肌肉瘤的发病机制中发挥重要作用。
