National Institute of Laser Enhanced Science (NILES), Cairo University, Egypt.
Talanta. 2012 Sep 15;99:140-5. doi: 10.1016/j.talanta.2012.05.031. Epub 2012 May 24.
The overall objective of this paper is to use a fast, more sensitive and less costly spectrochemical analysis laser techniques for estimation of seasonal variation of elements present in seminal plasma as well as for semen sperm count. For these two tasks we used Laser Induced-Breakdown Spectroscopy (LIBS) as an elemental analysis technique and Laser Induced Fluorescence (LIF) as a molecular analysis technique for sperm count estimation. The samples investigated via both techniques were buffalo semen from the artificial insemination center at the faculty of agriculture. The obtained LIBS data helped to assess indirectly the semen quality, sperm motility and spermatozoa count, relevant to the studied elements in different seasons. In addition it has been demonstrated that LIF can be adopted directly in centers of artificial insemination as a simple and fast method for the essential step of semen counting instead of the lengthy and inaccurate conventional techniques.
本文的总体目标是使用快速、更灵敏、成本更低的光谱化学分析激光技术来估算精液等离子体中元素的季节性变化以及精液精子计数。对于这两个任务，我们使用激光诱导击穿光谱（LIBS）作为元素分析技术，以及激光诱导荧光（LIF）作为精子计数估计的分子分析技术。通过这两种技术研究的样本是来自农业学院人工授精中心的水牛精液。获得的 LIBS 数据有助于间接评估精液质量、精子活力和精子计数，这与不同季节的研究元素有关。此外，已经证明 LIF 可以直接在人工授精中心采用，作为精液计数这一重要步骤的简单快速方法，而不是传统的冗长且不准确的技术。
