National Institute of Laser Enhanced Science (NILES) Cairo University, Egypt.
Theriogenology. 2011 Apr 1;75(6):988-94. doi: 10.1016/j.theriogenology.2010.11.005. Epub 2011 Jan 8.
The overall objective of this paper is to develop a more sensitive and less costly technique of laser irradiation of spermatozoa at certain wavelengths and exposure times suitable for improvement of buffalo semen quality. A 532 nm continuous wave (CW) DPSS laser light has been used to irradiate buffalo semen for different time intervals. Three semen pools from three different bulls (Bubalus bubalis) were used in the experiment, each pool was divided into six groups : control (not irradiated), and the other five were exposed to laser light for 1, 2, 3, 4 and 5 minutes with fluencies of 0.076, 0.15, 0.23, 0.31, and 0.38 Joule/cm² respectively at an output power 1mW. The results show that the semen quality parameters increase under the effect of laser irradiation. Maximum improvement in the semen quality has been reached after 4 minutes of exposure. Such results indicate the possibility of adopting laser irradiation as an easy and straightforward technique for in situ improvement of the semen quality to optimize the artificial insemination conditions.
本文的总体目标是开发一种更敏感、成本更低的激光辐照精子技术，选择合适的波长和辐照时间，以提高水牛精液质量。使用 532nm 连续波（CW）DPSS 激光照射水牛精液不同的时间间隔。该实验使用了来自三头不同公牛（Bubalus bubalis）的三个精液池，每个精液池分为六组：对照组（未辐照），其余五组分别用 0.076、0.15、0.23、0.31 和 0.38Joule/cm² 的通量在 1mW 的输出功率下辐照 1、2、3、4 和 5 分钟。结果表明，激光辐照对精液质量参数有提高作用。辐照 4 分钟后精液质量达到最大改善。这些结果表明，激光辐照有可能作为一种简单直接的原位改善精液质量的技术，以优化人工授精条件。
