IMAGES, EA 4218, Batiment S, Université de Perpignan, 52 avenue Paul Alduy 66 860, Perpignan Cedex, France.
Talanta. 2012 Sep 15;99:232-7. doi: 10.1016/j.talanta.2012.05.045. Epub 2012 May 30.
An electrochemical immunosensor for okadaic acid (OA) detection has been developed, and used in an indirect competitive immunoassay format under automated flow conditions. The biosensor was fabricated by injecting OA modified magnetic beads onto screen printed carbon electrode (SPCE) in the flow system. The OA present in the sample competed with the immobilized OA to bind with anti-okadaic acid monoclonal antibody (anti-OA-MAb). The secondary alkaline phosphatase labeled antibody was used to perform electrochemical detection. The current response obtained from the labeled alkaline phosphatase to 1-naphthyl phosphate decreased proportionally to the concentration of free OA in the sample. The calculated limit of detection (LOD) was 0.15 μg/L with a linear range of 0.19-25 μg/L. The good recoveries percentages validated the immunosensor application for real mussel samples. The developed system automatically controlled the incubation, washing and current measurement steps, showing its potential use for OA determination in field analysis.
一种用于检测 okadaic 酸（OA）的电化学免疫传感器已经被开发出来，并在自动化流动条件下以间接竞争免疫分析的形式使用。该生物传感器是通过在流动系统中将 OA 修饰的磁性珠注入到丝网印刷碳电极（SPCE）上来制备的。样品中的 OA 与固定化 OA 竞争与抗 okadaic 酸单克隆抗体（抗-OA-MAb）结合。然后使用碱性磷酸酶标记的抗体进行电化学检测。标记的碱性磷酸酶对 1-萘基磷酸的电流响应与样品中游离 OA 的浓度成比例地降低。计算得到的检测限（LOD）为 0.15μg/L，线性范围为 0.19-25μg/L。良好的回收率验证了该免疫传感器在实际贻贝样品中的应用。所开发的系统自动控制孵育、洗涤和电流测量步骤，表明其在现场分析中用于 OA 测定的潜力。
