Departamento de Química, Universidade Federal de Lavras (UFLA), CP 3037, Lavras, CEP 37200-000 MG, Brazil.
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000 São Paulo, SP, Brazil.
Sensors (Basel). 2018 Nov 24;18(12):4124. doi: 10.3390/s18124124.
Disposable immunosensors are analytical devices used for the quantification of a broad variety of analytes in different areas such as clinical, environmental, agricultural and food quality management. They detect the analytes by means of the strong interactions between antibodies and antigens, which provide concentration-dependent signals. For the herein highlighted voltammetric immunosensors, the analytical measurements are due to changes in the electrical signals on the surface of the transducers. The possibility of using disposable and miniaturized immunoassays is a very interesting alternative for voltammetric analyses, mainly, when associated with screen-printing technologies (screen-printed electrodes, SPEs), and microfluidic platforms. The aim of this paper is to discuss a carefully selected literature about different examples of SPEs-based immunosensors associated with microfluidic technologies for diseases, food, agricultural and environmental analysis. Technological aspects of the development of the voltammetric immunoassays such as the signal amplification, construction of paper-based microfluidic platforms and the utilization of microfluidic devices for point-of-care testing will be presented as well.
一次性免疫传感器是用于分析不同领域（如临床、环境、农业和食品质量控制）中各种分析物的分析设备。它们通过抗体和抗原之间的强相互作用来检测分析物，提供与浓度相关的信号。对于此处突出显示的伏安免疫传感器，分析测量是由于传感器表面的电信号发生变化。使用一次性和微型化免疫分析的可能性是伏安分析的一个非常有趣的替代方案，特别是当与丝网印刷技术（丝网印刷电极，SPE）和微流控平台结合使用时。本文的目的是讨论有关与微流控技术结合用于疾病、食品、农业和环境分析的基于 SPE 的免疫传感器的不同示例的精选文献。还将介绍伏安免疫分析的发展的技术方面，例如信号放大、基于纸张的微流控平台的构建以及微流控设备在即时检测中的利用。
