Department of Analytical Chemistry, University of Valencia Edificio Jerónimo Muñoz, 50th Dr. Moliner, 46100 Burjassot, Spain.
Talanta. 2012 Sep 15;99:660-7. doi: 10.1016/j.talanta.2012.06.072. Epub 2012 Jun 29.
A fast, reliable and economical methodology has been developed to control the production process of sugar-based depilatories. The method is based on the use of attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy in combination with multivariate data analysis. A very simple sample preparation process involving the dissolution of samples in water was applied. Employing a multivariate calibration model established from data of 15 well characterized samples, prediction errors equal or below 3.04 mg mL(-1) for the quantitative determination of fructose, glucose, sucrose, maltose and maltotriose were obtained. Results found in this preliminary study indicate a great potential for the development of at-line ATR-FTIR-PLS methods based on a careful selection of variables from IR spectra, delivering fast and reliable results. As a reference method, a liquid chromatography (LC)-IR method was adapted for sample characterization.
已开发出一种快速、可靠且经济的方法来控制基于糖的脱毛剂的生产过程。该方法基于衰减全反射傅里叶变换红外（ATR-FTIR）光谱与多元数据分析相结合的原理。应用了一种非常简单的样品制备方法，涉及将样品溶解在水中。通过使用从 15 个特征良好的样品数据建立的多元校准模型，对果糖、葡萄糖、蔗糖、麦芽糖和麦芽三糖的定量测定，预测误差等于或低于 3.04mg mL(-1)。本初步研究结果表明，基于从 IR 光谱中仔细选择变量，开发在线 ATR-FTIR-PLS 方法具有很大的潜力，可提供快速可靠的结果。作为参考方法，对液相色谱（LC）-IR 方法进行了调整，以用于样品表征。
