傅里叶变换红外衰减全反射光谱法在蜂蜜中糖分定量分析中的应用。
Application of FTIR-ATR spectroscopy to the quantification of sugar in honey.
作者信息
Anjos Ofélia, Campos Maria Graça, Ruiz Pablo Contreras, Antunes Paulo
机构信息
IPCB/ESA - Instituto Politécnico de Castelo Branco, Escola Superior Agrária, 6001-909 Castelo Branco, Portugal; CEF, Universidade de Lisboa, ISA, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Drug Discovery Group, Center for Pharmaceutical Studies, Faulty of Pharmacy, University of Coimbra, Health Sciences Campus, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.
出版信息
Food Chem. 2015 Feb 15;169:218-23. doi: 10.1016/j.foodchem.2014.07.138. Epub 2014 Aug 10.
A Fourier transform infrared spectroscopic method with attenuated total reflectance (FTIR-ATR) and partial least squares (PLS) regression model for the prediction of sugar content in honey samples was calculated. Standards of trehalose, glucose, fructose, sucrose, melezitose, turanose and maltose were used to identify and quantify the individual sugar components in 63 honey samples by HPAEC-IPAD. Fructose and glucose are the highest sugars in honey with an average value of 36% and 26%, respectively. The 1stDer spectra with MSC or SLS in the wave number range from 1500 to 750cm(-1) provide the best calibration model with a r(2) of 86.60 and 86.01 with RPD of 2.6 and 2.55, respectively for fructose and glucose. For turanose and melezitose good models were also found. The FTIR-ATR showed to be a good methodology to quantify the main sugar content in honey and easily adapted to routine analysis.
计算了一种采用衰减全反射傅里叶变换红外光谱法（FTIR-ATR）和偏最小二乘法（PLS）回归模型预测蜂蜜样品中糖分含量的方法。使用海藻糖、葡萄糖、果糖、蔗糖、松三糖、松二糖和麦芽糖标准品，通过高效阴离子交换色谱-积分脉冲安培检测法（HPAEC-IPAD）对63个蜂蜜样品中的各糖分成分进行鉴定和定量。果糖和葡萄糖是蜂蜜中含量最高的糖类，平均值分别为36%和26%。在波数范围1500至750cm⁻¹内具有多元散射校正（MSC）或标准归一化变换（SLS）的一阶导数光谱提供了最佳校准模型，果糖和葡萄糖的决定系数（r²）分别为86.60和86.01，相对分析误差（RPD）分别为2.6和2.55。对于松二糖和松三糖也发现了良好的模型。FTIR-ATR显示是一种定量蜂蜜中主要糖分含量的良好方法，且易于应用于常规分析。
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