大环单端孢霉烯同系物对小鼠脾淋巴细胞活力和有丝分裂的影响。

Effects of macrocyclic trichothecene congeners on the viability and mitogenesis of murine splenic lymphocytes.

作者信息

Hughes B J, Jarvis B B, Sharma R P

机构信息

Toxicology Program, Utah State University, Logan 84322-5600.

出版信息

Toxicol Lett. 1990 Jan;50(1):57-67. doi: 10.1016/0378-4274(90)90252-h.

DOI:10.1016/0378-4274(90)90252-h

PMID:2296778

Abstract

The effects of several congeners of the macrocyclic class of trichothecene mycotoxins on murine splenic cells in vitro were investigated. The mycotoxins were roritoxin B, myrotoxin B, roridins A, D and E, baccharinoids B4, B5 and B12, 16-hydroxyverrucarin A, and verrucarins A and J. Lymphocytes from CD-1 mice were cultured with each of the mycotoxins for 48 h to assess cytotoxicity. The maximum effect of various trichothecenes produced on cells occurred at concentrations ranging from 10(-6) to 10(-4) M. Mycotoxins had no effect at concentrations ranging from 10(-12) to 10(-7) M. The mitogenic stimulants concanavalin A, lipopolysaccharide, phytohemagglutinin, and pokeweed mitogen were added to splenic lymphocyte cultures along with varying concentrations of selected mycotoxins. Blastogenesis was inhibited at concentrations 2-5 orders of magnitude lower than those which produced lethality on resting lymphocytes.

摘要

研究了大环类单端孢霉烯族霉菌毒素的几种同系物对体外培养的小鼠脾细胞的影响。这些霉菌毒素包括roritoxin B、myrotoxin B、roridins A、D和E、baccharinoids B4、B5和B12、16 - 羟基疣孢菌素A以及疣孢菌素A和J。将来自CD - 1小鼠的淋巴细胞与每种霉菌毒素培养48小时以评估细胞毒性。各种单端孢霉烯族毒素对细胞产生的最大效应出现在10^(-6)至10^(-4) M的浓度范围内。霉菌毒素在10^(-12)至10^(-7) M的浓度范围内无作用。将促有丝分裂刺激剂刀豆球蛋白A、脂多糖、植物血凝素和商陆有丝分裂原与不同浓度的选定霉菌毒素一起添加到脾淋巴细胞培养物中。在比导致静息淋巴细胞致死的浓度低2 - 5个数量级的浓度下，细胞增殖受到抑制。