Havenith M G, Dingemans K P, Cleutjens J P, Wagenaar S S, Bosman F T
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Ultrastruct Pathol. 1990 Jan-Feb;14(1):51-63. doi: 10.3109/01913129009050874.
Basement membrane (BM) deposition at the inter-face of tumor cells and stroma was studied in 27 bronchogenic squamous cell carcinomas. Specimens from peripheral and central parts of each tumor were collected. These were frozen, formalin fixed and paraffin embedded or fixed in Karnovsky's fixative, and processed for electron microscopy. With the use of antibodies to type IV collagen and laminin, the BM was visualized by light microscopy with an indirect immunoperoxidase technique. Light microscopic findings were compared to ultrastructural observations. The peripheral parts of the tumors showed continuous BM in a recognizable preexisting alveolar pattern without evidence of invasive growth into the alveolar septa. In contrast, central parts showed highly variable BM deposition ranging from continuous to almost completely absent. Alveolar patterns were not observed in the tumor centers. The stromal compartment of the tumor centers contained many spindle cells with irregular pericellular BM-like material that could be identified ultrastructurally as myofibroblasts. Electron microscopy and immunohistochemistry yielded virtually identical results. It is concluded that invasive growth in bronchogenic squamous cell carcinomas occurs in central parts of the tumor when the tumor periphery shows expansive growth without invasion of alveolar septa. The situation is different in invasive squamous cell carcinomas originating from other organs because of anatomical differences between the lung and solid organs.
在27例支气管源性鳞状细胞癌中研究了肿瘤细胞与基质界面处基底膜（BM）的沉积情况。收集了每个肿瘤外周和中央部分的标本。这些标本被冷冻、用福尔马林固定并石蜡包埋，或固定于卡诺夫斯基固定液中，然后进行电子显微镜处理。使用抗IV型胶原和层粘连蛋白的抗体，通过间接免疫过氧化物酶技术在光学显微镜下观察基底膜。将光学显微镜下的观察结果与超微结构观察结果进行比较。肿瘤外周部分显示基底膜呈可识别的原有肺泡模式连续存在，无侵犯肺泡间隔的证据。相反，中央部分基底膜沉积变化很大，从连续到几乎完全缺失。在肿瘤中央未观察到肺泡模式。肿瘤中央的基质区含有许多梭形细胞，其周围有不规则的类似基底膜的物质，超微结构可鉴定为肌成纤维细胞。电子显微镜和免疫组织化学得出了几乎相同的结果。得出的结论是，当肿瘤外周显示膨胀性生长而未侵犯肺泡间隔时，支气管源性鳞状细胞癌的侵袭性生长发生在肿瘤中央部分。由于肺与实体器官的解剖学差异，起源于其他器官的浸润性鳞状细胞癌情况不同。
