Dingemans K P, Mooi W J
Int J Cancer. 1986 Jan 15;37(1):11-9. doi: 10.1002/ijc.2910370104.
We collected multiple samples from 43 human bronchogenic squamous-cell carcinomas and studied the ultrastructure of the tumor-cell-lung parenchyma interaction in the tumor periphery. Since, in the periphery of a growing tumor, the surrounding tissue has only recently been reached by the tumor cells, it is the initial stage of interaction which can be observed here. The main findings were: The tumor cells in the tumor periphery always penetrated the lung parenchyma along the epithelial side of the alveolar basal lamina. The non-neoplastic alveolar epithelial cells were either detached from their basal lamina or overgrown by the tumor cells, without being visibly damaged or destroyed. When the alveolar epithelial cells were overgrown by the tumor, they retracted and formed extra- or intracellular lumina much smaller than the original alveoli. The contact between tumor cells and alveolar epithelial cells resulted in the formation of common desmosomes and complete junctional complexes and the common lining of lumina. Although the tumor cells extended small pseudopods through the basal lamina, they virtually never migrated through it to reach the interstitial compartment. These results indicate that the initial invasion of lung parenchyma is characterized by a smooth integration of tumor cells and elements of the preexisting tissue, leading to orderly associations of neoplastic and non-neoplastic cells, rather than by tissue destruction by the tumor cells.
我们从43例人支气管源性鳞状细胞癌中采集了多个样本，并研究了肿瘤周边肿瘤细胞与肺实质相互作用的超微结构。由于在生长中的肿瘤周边，周围组织最近才刚刚被肿瘤细胞累及，所以这里可以观察到相互作用的初始阶段。主要发现如下：肿瘤周边的肿瘤细胞总是沿着肺泡基底膜的上皮侧穿透肺实质。非肿瘤性肺泡上皮细胞要么从其基底膜脱离，要么被肿瘤细胞过度生长，且未见明显损伤或破坏。当肺泡上皮细胞被肿瘤过度生长时，它们会回缩并形成比原始肺泡小得多的细胞外或细胞内腔隙。肿瘤细胞与肺泡上皮细胞之间的接触导致形成共同的桥粒和完整的连接复合体以及腔隙的共同内衬。尽管肿瘤细胞通过基底膜伸出小伪足，但它们实际上从未穿过基底膜到达间质间隙。这些结果表明，肺实质的初始侵袭特征是肿瘤细胞与原有组织成分的平滑整合，导致肿瘤细胞与非肿瘤细胞有序关联，而非肿瘤细胞对组织的破坏。
