Department of Cornea and Ocular Surface, Institut Catala de la Retina, Barcelona, Spain.
Cornea. 2012 Dec;31(12):1489-92. doi: 10.1097/ICO.0b013e31825d5853.
To determine how the air bubble dissects Descemet membrane from the inside during experimental deep lamellar anterior keratoplasty.
In this experimental study, 4 corneas from enucleated human eyes were dissected and placed in an artificial anterior chamber. Air was injected through a 27-gauge needle into the midperiphery without previous trephination until a big bubble formed. The results were photographed from the inside.
The edge of the air dissection was viewed clearly. The dissection was not uniform; one side was dissected more than the other. Descemet membrane and the stroma may still have been attached. The results provided a clue about why perforation frequently occurs when dissecting the anterior lamella in the periphery.
As much air as possible should be injected. Premature cessation of the injection of air can result in irregular dissection by all edges of the bubble or an incomplete bubble.
在实验性深层板层角膜移植术中，确定气泡如何从内部将角膜内皮从角膜内分离。
在这项实验研究中，将 4 个来自眼球摘除的人眼角膜进行解剖并放置在人工前房中。通过 27 号针头从前部周边注入空气，直到形成一个大气泡，无需预先环钻。结果从内部拍照。
可以清楚地看到气泡分离的边缘。分离不均匀；一侧比另一侧分离得多。角膜内皮和基质可能仍然相连。该结果提供了一个线索，说明为什么在前部周边进行前板层解剖时经常发生穿孔。
应尽可能多地注入空气。过早停止注入空气可能会导致气泡的所有边缘不规则分离或气泡不完整。
