UMR 6022 Laboratoire de Génie Enzymatique et Cellulaire, Université de Technologie de Compiègne-CNRS, Compiègne, France.
Anal Bioanal Chem. 2013 Feb;405(5):1445-61. doi: 10.1007/s00216-012-6383-y. Epub 2012 Sep 12.
Supported lipid bilayers (SLBs) are biomimetic model systems that are now widely used to address the biophysical and biochemical properties of biological membranes. Two main methods are usually employed to form SLBs: the transfer of two successive monolayers by Langmuir-Blodgett or Langmuir-Schaefer techniques, and the fusion of preformed lipid vesicles. The transfer of lipid films on flat solid substrates offers the possibility to apply a wide range of surface analytical techniques that are very sensitive. Among them, atomic force microscopy (AFM) has opened new opportunities for determining the nanoscale organization of SLBs under physiological conditions. In this review, we first focus on the different protocols generally employed to prepare SLBs. Then, we describe AFM studies on the nanoscale lateral organization and mechanical properties of SLBs. Lastly, we survey recent developments in the AFM monitoring of bilayer alteration, remodeling, or digestion, by incubation with exogenous agents such as drugs, proteins, peptides, and nanoparticles.
支持脂质双层 (SLB) 是仿生模型系统，现在广泛用于解决生物膜的生物物理和生化特性。形成 SLB 通常采用两种主要方法：通过 Langmuir-Blodgett 或 Langmuir-Schaefer 技术转移两个连续的单层，以及预形成的脂质囊泡融合。在平坦的固体基底上转移脂质膜提供了应用广泛的非常敏感的表面分析技术的可能性。其中，原子力显微镜 (AFM) 为在生理条件下确定 SLB 的纳米级组织提供了新的机会。在这篇综述中，我们首先重点介绍了通常用于制备 SLB 的不同方案。然后，我们描述了 AFM 对 SLB 的纳米级横向组织和机械性能的研究。最后，我们调查了通过与外源性试剂（如药物、蛋白质、肽和纳米颗粒）孵育，AFM 监测双层改变、重塑或消化的最新进展。
