Department of Chemistry, Marquette University, Milwaukee, WI 53233, USA.
Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10403-7. doi: 10.1002/anie.201205912. Epub 2012 Sep 11.
Resonance Raman spectroscopy is used to document, for the first time, a 6 cm decrease of the Fe-S stretch by introducing an H-bond donor into the proximal pocket of a cytochrome P450, which interacts with the key cysteine thiolate axial ligand. The anticipated trans-effect on bound exogenous ligands is also confirmed and evidence is obtained supporting intimate interaction of the new histidyl-imidazole fragment with the heme periphery.
共振拉曼光谱首次被用于记录,通过在细胞色素 P450 的近端口袋中引入氢键供体,Fe-S 伸缩振动减少了 6 厘米,该口袋与关键的半胱氨酸硫醇轴向配体相互作用。对外源配体的预期反式效应也得到了证实,并获得了支持新的组氨酸咪唑片段与血红素外围密切相互作用的证据。