Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea.
J Korean Med Sci. 2012 Sep;27(9):1009-13. doi: 10.3346/jkms.2012.27.9.1009. Epub 2012 Aug 22.
Recently, subpopulations of regulatory T (Treg) cells, resting Treg (rTreg) and activated Treg (aTreg), have been discovered. The authors investigated the relationship between the change of Treg, aTreg and rTreg and autoimmune diseases. Treg cells and those subpopulations were analyzed by using the human regulatory T cell staining kit and CD45RA surface marker for 42 rheumatoid arthritis (RA), 13 systemic lupus sclerosis (SLE), 7 Behcet's disease (BD), and 22 healthy controls. The proportion of Treg cells was significantly lower in RA (3.8% ± 1.0%) (P < 0.001) and BD (3.3% ± 0.5%) (P < 0.01) compared to healthy controls (5.0% ± 1.3%). The proportion of aTreg cells was also significantly lower in RA (0.4% ± 0.2%) (P = 0.008) and BD (0.3% ± 0.1%) (P = 0.013) compared to healthy controls (0.6% ± 0.3%). The rTreg cells showed no significant differences. The ratio of aTreg to rTreg was lower in RA patients (0.4% ± 0.2%) than that in healthy controls (0.7% ± 0.4%) (P = 0.002). This study suggests that the decrement of aTreg not rTreg cells contributes the decrement of total Treg cells in peripheral blood of RA and BD autoimmune diseases. Detailed analysis of Treg subpopulations would be more informative than total Treg cells in investigating mechanism of autoimmune disease.
最近，人们发现了调节性 T（Treg）细胞的亚群，包括静息 Treg（rTreg）和活化 Treg（aTreg）。作者研究了 Treg、aTreg 和 rTreg 变化与自身免疫性疾病之间的关系。使用人调节性 T 细胞染色试剂盒和 CD45RA 表面标志物，对 42 例类风湿关节炎（RA）、13 例系统性红斑狼疮（SLE）、7 例白塞病（BD）和 22 例健康对照者的 Treg 细胞和这些亚群进行了分析。与健康对照组（5.0%±1.3%）相比，RA（3.8%±1.0%）（P<0.001）和 BD（3.3%±0.5%）（P<0.01）患者的 Treg 细胞比例明显降低。RA（0.4%±0.2%）（P=0.008）和 BD（0.3%±0.1%）（P=0.013）患者的 aTreg 细胞比例也明显低于健康对照组（0.6%±0.3%）。rTreg 细胞无显著差异。RA 患者 aTreg/rTreg 比值（0.4%±0.2%）低于健康对照组（0.7%±0.4%）（P=0.002）。本研究表明，RA 和 BD 自身免疫性疾病患者外周血中总 Treg 细胞减少是由于 aTreg 细胞而不是 rTreg 细胞减少所致。与总 Treg 细胞相比，Treg 亚群的详细分析可能更有助于研究自身免疫性疾病的发病机制。
