Moon Hee-Won, Kim Bo Hyun, Park Chul Min, Hur Mina, Yun Yeo-Min, Kim Sung-Yong, Lee Mark Hong
Department of Laboratory Medicine, Konkuk University School of Medicine, Hospital, 4-12 Hwayang-dong, Gwangjin-gu, Seoul, Korea.
Korean J Lab Med. 2011 Oct;31(4):231-7. doi: 10.3343/kjlm.2011.31.4.231. Epub 2011 Oct 3.
CD4+CD25+ regulatory T-cells (Tregs) play a critical role in immune responses. We explored the status of Tregs in neoplastic and autoimmune hematologic diseases. We also evaluated the technical aspects of Treg measurement in terms of sample type and detection markers.
A total of 68 subjects were enrolled: 11 with AML, 8 with MDS, 10 with autoimmune diseases, and 39 controls. Tregs were analyzed in peripheral blood (PB) and bone marrow (BM) samples from each subject. Flow cytometry and the Human Regulatory T cell Staining Kit (eBioscience, USA) for CD4, CD25, and FoxP3 (forkhead box P3) were used.
The CD4+CD25(high)/CD4 and CD4+CD25(high)FoxP3+/CD4 populations were significantly correlated (P<0.0001). The AML and high-risk MDS groups had significantly larger CD4+CD25(high)/CD4 and CD4+CD25(high)FoxP3+/CD4 populations in PB than the autoimmune (P=0.007 and 0.012, respectively) and control groups (P=0.004 and 0.006, respectively). Comparable findings were observed in BM. The CD4+CD25(high)FoxP3+/CD4 population was significantly larger in PB than in BM (P=0.0003).
This study provides comparison data for Tregs in AML, MDS, and autoimmune hematologic diseases, and would be helpful for understanding the different immunologic bases of various hematologic diseases. Treg measurement using CD4, CD25, and/or FoxP3 in PB rather than in BM seems to be practical for routine hematologic purposes. Large-scale analysis of the diagnostic role of Treg measurement is needed.
CD4+CD25+调节性T细胞（Tregs）在免疫反应中起关键作用。我们探讨了Tregs在肿瘤性和自身免疫性血液疾病中的状态。我们还从样本类型和检测标志物方面评估了Treg测量的技术问题。
共纳入68名受试者：11例急性髓系白血病（AML）患者、8例骨髓增生异常综合征（MDS）患者、10例自身免疫性疾病患者和39名对照者。对每个受试者的外周血（PB）和骨髓（BM）样本中的Tregs进行分析。使用流式细胞术和用于检测CD4、CD25和FoxP3（叉头框P3）的人类调节性T细胞染色试剂盒（美国eBioscience公司）。
CD4+CD25（高表达）/CD4和CD4+CD25（高表达）FoxP3+/CD4群体显著相关（P<0.0001）。AML组和高危MDS组外周血中CD4+CD25（高表达）/CD4和CD4+CD25（高表达）FoxP3+/CD4群体显著大于自身免疫性疾病组（分别为P=0.007和0.012）和对照组（分别为P=0.004和0.006）。在骨髓中观察到类似结果。外周血中CD4+CD25（高表达）FoxP3+/CD4群体显著大于骨髓（P=0.0003）。
本研究提供了AML、MDS和自身免疫性血液疾病中Tregs的比较数据，有助于理解各种血液疾病不同的免疫基础。在PB中而非BM中使用CD4、CD25和/或FoxP3测量Tregs似乎对常规血液学检查实用。需要对Treg测量的诊断作用进行大规模分析。
