Li Jun, Wang Haifeng, Ma Zhen, Fan Wenxing, Li Yanfeng, Han Bingbing, Zhang Zhijia, Wang Jiansong
Department of Nephrology, The First Affiliated Hospital, Kunming Medical University, Kunming 650031;
Exp Ther Med. 2012 Jun;3(6):1033-1038. doi: 10.3892/etm.2012.520. Epub 2012 Mar 21.
In order to identify the antitumor effect of TAT-Apoptin on the human bladder cancer EJ cell line and study its impact on the expression of the apoptosis-related genes bax, bcl-2, caspase-3 and survivin, the MTT assay, real-time quantitative PCR and western blot analysis were used in this study. The results of the MTT assay indicated that TAT-Apoptin was able to inhibit the proliferation of EJ cells in a dose-dependent manner. The expression of Bax, Bcl-2, Caspase-3 and Survivin mRNA and protein following the treatment of the EJ cells with TAT-Apoptin (0.1, 0.5, 1, 10, 50 and 100 μg/ml) for 24, 48 and 72 h was analyzed. Cell proliferation was significantly different after treatment with the various concentrations of TAT-Apoptin and the durations of treatment. The level of expression of Bcl-2 and Survivin in EJ cells decreased significantly, while that of Bax and Caspase-3 increased significantly at the mRNA and protein levels.
为了确定TAT-凋亡素对人膀胱癌EJ细胞系的抗肿瘤作用，并研究其对凋亡相关基因bax、bcl-2、caspase-3和survivin表达的影响，本研究采用了MTT法、实时定量PCR和蛋白质印迹分析。MTT法结果表明，TAT-凋亡素能够以剂量依赖的方式抑制EJ细胞的增殖。分析了用TAT-凋亡素（0.1、0.5、1、10、50和100μg/ml）处理EJ细胞24、48和72小时后Bax、Bcl-2、Caspase-3和Survivin mRNA及蛋白质的表达。用不同浓度的TAT-凋亡素处理并经过不同处理时间后，细胞增殖存在显著差异。EJ细胞中Bcl-2和Survivin的表达水平在mRNA和蛋白质水平上显著降低，而Bax和Caspase-3的表达水平则显著升高。
