Chen L, Dong S-W, Tao X, Liu J-P, Tang K-L, Xu J-Z
Department of Orthopaedics, Southwest Hospital, and Department of Anatomy, Third Military Medical University, Chongqing, China.
J Int Med Res. 2012;40(4):1399-409. doi: 10.1177/147323001204000418.
To explore the effects of autologous platelet-rich clot releasate (PRCR) on proliferation and differentiation of adult rat tendon stem cells (TSCs) in vitro, following intense mechanical stretching.
TSCs were subjected to 8% mechanical stretching and subsequently incubated in control medium or medium supplemented with 2% or 10% PRCR. Collagen types I and III, peroxisome proliferator-activated receptor-γ (PPARγ), sex determining region Y-box 9 (SOX-9) and runt-related transcription factor 2 (RUNX2) concentrations were assessed via Western blotting and flow cytometry. Transforming growth factor (TGF)-β1 and vascular endothelial growth factor concentrations were measured using enzyme-linked immunosorbent assay. Treated TSCs were also cultured in adipogenic, chondrogenic or osteogenic culture media.
PRCR increased the number of TSCs, and the concentrations of collagen types I and III and TGF-β1. In contrast, PRCR significantly reduced PPARγ, SOX-9 and RUNX2-positive cell numbers, and significantly reduced the numbers of TSC-derived adipocytes, chondrocytes and osteocytes.
PRCR induced tenocyte differentiation while suppressing the adipocyte, chondrocyte and osteocyte lineages believed to impede tendon healing.
探讨自体富血小板凝块释放物（PRCR）对成年大鼠肌腱干细胞（TSCs）在体外受到强烈机械拉伸后增殖和分化的影响。
对TSCs施加8%的机械拉伸，随后在对照培养基或添加2%或10%PRCR的培养基中培养。通过蛋白质印迹法和流式细胞术评估I型和III型胶原蛋白、过氧化物酶体增殖物激活受体-γ（PPARγ）、性别决定区Y盒9（SOX-9）和 runt相关转录因子2（RUNX2）的浓度。使用酶联免疫吸附测定法测量转化生长因子（TGF）-β1和血管内皮生长因子的浓度。处理后的TSCs也在成脂、成软骨或成骨培养基中培养。
PRCR增加了TSCs的数量，以及I型和III型胶原蛋白和TGF-β1的浓度。相比之下，PRCR显著减少了PPARγ、SOX-9和RUNX2阳性细胞的数量，并显著减少了TSC衍生的脂肪细胞、软骨细胞和骨细胞的数量。
PRCR诱导肌腱细胞分化，同时抑制被认为会阻碍肌腱愈合的脂肪细胞、软骨细胞和骨细胞谱系。
