Kotani K, Tsuzaki K, Sakane N
Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Centre, 1-1 Fukakusa mukaihata, Fushimi-ku, Kyoto 612-8555, Japan.
J Int Med Res. 2012;40(4):1513-8. doi: 10.1177/147323001204000431.
Paraoxonase-1 (PON1) is a high-density lipoprotein-associated antioxidant enzyme. The Q192R polymorphism of the PON1 gene can protect against oxidative conditions, but the relationship between Q192R polymorphism and oxidative stress-related markers remains controversial. In this study, the diacron reactive oxygen metabolites (d-ROMs) test was used to investigate the relationship between Q192R polymorphism and oxidative stress-related markers in Japanese subjects.
Patients without a history of overt cardiovascular disease who were not receiving antioxidant medication were enrolled in a cross-sectional clinic-based study. An allele-specific polymerase chain reaction method was used to assess the PON1 Q192R polymorphism and compare the level of d-ROMs between genotypes.
A total of 103 subjects were analysed. The RR genotype was associated with a significantly lower level of d-ROMs than the RQ and QQ genotypes. After multivariate analysis the relationship between the genotypes and level of d-ROMs remained independently significant.
The RR genotype may be protective against oxidative stress in cardiovascular diseasefree Japanese subjects. In addition, the d-ROMs test can be useful for examining the role of the PON1 Q192R polymorphism under oxidative conditions.
对氧磷酶-1（PON1）是一种与高密度脂蛋白相关的抗氧化酶。PON1基因的Q192R多态性可抵御氧化状态，但Q192R多态性与氧化应激相关标志物之间的关系仍存在争议。在本研究中，采用戴克隆活性氧代谢产物（d-ROMs）检测来研究日本受试者中Q192R多态性与氧化应激相关标志物之间的关系。
将无明显心血管疾病病史且未接受抗氧化药物治疗的患者纳入一项基于门诊的横断面研究。采用等位基因特异性聚合酶链反应方法评估PON1 Q192R多态性，并比较各基因型之间的d-ROMs水平。
共分析了103名受试者。RR基因型与d-ROMs水平显著低于RQ和QQ基因型相关。多变量分析后，基因型与d-ROMs水平之间的关系仍具有独立显著性。
RR基因型可能对无心血管疾病的日本受试者的氧化应激具有保护作用。此外，d-ROMs检测对于研究氧化条件下PON1 Q192R多态性的作用可能有用。
