Hogendorf Anna, Stańczyk-Przyłuska Anna, Siniewicz-Luzeńczyk Katarzyna, Wiszniewska Magdalena, Arendarczyk Jerzy, Banasik Małgorzata, Fendler Wojciech, Kowalski Marek, Zeman Krzysztof
Klinika Pediatrii, Onkologii, Hematologii i Diabetologii Uniwersytetu Medycznego w Łodzi, ul. Sporna 36/50, Łódź, Poland.
Med Wieku Rozwoj. 2012 Apr-Jun;16(2):104-8.
The aim of the study was to determine the concentration of such immunomodulating factors as transforming growth factor beta1 (TGF-β1), interleukin 10 (IL-10), tumor necrosis factor alpha (TNF-α) in mature human milk and to relate the levels of the above mentioned cytokines in mature breast milk to the occurence of food allergy in children during the first 24 months of life.
Data on breastfeeding, symptoms of food allergy in children and breast milk samples were collected prospectively from birth to 24 months of age from 84 mothers participating in the Polish birth cohort of "EuroPrevall" study, in the years 2005-2007. Cytokine levels were measured in the whey with commercial enzyme-linked immunosorbent assays (ELISA) kits.
Ten out of the eighty four (11.9%) participating children had positive SPT and/or sIgE to food antigens. In 7 out of 84 (8.4%) children DBPCFC confirmed the diagnosis of food allergy. The median concentration of TGF-β1 was 21.94 pg/ml (range 10.47-83.19), TNF-α 1.46 pg/ml (range 0.35-16.50), IL- 101.83 pg/ml (range 0.58-31.04). There was a positive correlation between the concentration of IL-10 and TGF-β1. The level of TNF-α correlated positively with the duration of lactation (p=0.04). There was no significant difference between the concentration of IL-10, TGF-β1, TNF-α, in the mature breast milk of mothers of children with symptoms of allergy and positive SPT and/or sIgE, mothers of children with positive DBPCFC and in the milk of mothers of control children.
There was no significant difference between the concentration of IL-10, TGF-β1, TNF-α, in the mature breast milk of mothers of children with food allergy and in the breast milk of mothers of control children.
本研究旨在测定成熟人乳中转化生长因子β1（TGF-β1）、白细胞介素10（IL-10）、肿瘤坏死因子α（TNF-α）等免疫调节因子的浓度，并将成熟母乳中上述细胞因子的水平与儿童出生后头24个月内食物过敏的发生情况相关联。
2005年至2007年期间，前瞻性收集了参与“欧洲Prevail”研究波兰出生队列的84名母亲从孩子出生到24个月大的母乳喂养、儿童食物过敏症状及母乳样本数据。采用商业酶联免疫吸附测定（ELISA）试剂盒测定乳清中的细胞因子水平。
84名参与研究的儿童中，有10名（11.9%）对食物抗原的皮肤点刺试验（SPT）和/或特异性免疫球蛋白E（sIgE）呈阳性。84名儿童中有7名（8.4%）通过双盲安慰剂对照食物激发试验（DBPCFC）确诊为食物过敏。TGF-β1的中位浓度为21.94 pg/ml（范围10.47 - 83.19），TNF-α为1.46 pg/ml（范围0.35 - 16.50），IL-10为1.83 pg/ml（范围0.58 - 31.04）。IL-10和TGF-β1的浓度之间存在正相关。TNF-α的水平与哺乳时间呈正相关（p = 0.04）。有过敏症状且SPT和/或sIgE呈阳性的儿童母亲的成熟母乳、DBPCFC呈阳性的儿童母亲的母乳以及对照儿童母亲的母乳中，IL-10、TGF-β1、TNF-α的浓度之间无显著差异。
食物过敏儿童母亲的成熟母乳中IL-１０、TGF-β1、TNF-α的浓度与对照儿童母亲的母乳中的浓度无显著差异。
