Degawa Takuma, Shimma Shuichi, Toyoda Michisato
School of Science, Osaka University, Toyonaka, Osaka, Japan.
Eur J Mass Spectrom (Chichester). 2012;18(4):345-8. doi: 10.1255/ejms.1188.
A peptide containing a single disulfide bond was sequenced using high-energy collision-induced dissociation (HE-CID) in conjunction with a high mass resolution time-of-flight tandem mass spectrometer equipped with a matrix-assisted laser desorption/ionization source. This mass spectrometer, which has spiral ion trajectory, allowed both high mass resolution and high precursor ion selectivity. It is difficult to obtain sufficient product ions from peptides containing disulfide bonds using HE-CID due to the single collision in the gas phase. To compensate for insufficient dissociation, the disulfide bond was cleaved via an in-source reduction process using 1,5-diaminonaphthalene, a reducing matrix. After applying the reduction in the ionization, subsequent sequencing using HE-CID provided the detailed structural information of the peptide containing the single disulfide bond.
使用配备基质辅助激光解吸/电离源的高质量分辨率飞行时间串联质谱仪，结合高能碰撞诱导解离（HE-CID）对含有单个二硫键的肽段进行测序。这种具有螺旋离子轨迹的质谱仪，既实现了高质量分辨率，又具备高前体离子选择性。由于气相中的单次碰撞，使用HE-CID难以从含有二硫键的肽段中获得足够的产物离子。为了弥补解离不足，通过使用还原基质1,5-二氨基萘的源内还原过程裂解二硫键。在电离过程中进行还原后，随后使用HE-CID进行测序，提供了含有单个二硫键的肽段的详细结构信息。
