Li Gongyu, Pei Jiying, Yin Yue, Huang Guangming
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.
Analyst. 2015 Apr 21;140(8):2623-7. doi: 10.1039/c5an00011d.
Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.
在通过电喷雾电离碰撞诱导解离质谱法（ESI-CID-MS）对二硫键肽进行测序之前，二硫键的解离通常是必不可少的。在此，我们提出了一种直接对完整二硫键肽进行测序的简便方法。其中涉及的基本原理是电喷雾电离中的电解质增强电晕放电以及随后的氧化裂解反应。
