School of Pharmacy, University of Oslo, 0316 Oslo, Norway.
J Ethnopharmacol. 2012 Oct 31;144(1):82-5. doi: 10.1016/j.jep.2012.08.031. Epub 2012 Sep 3.
Roots from Vernonia kotschyana are on the national list of essential drugs in Mali (West-Africa). It is sold under the name Gastrosedal and it used against ailments like gastritis and gastric ulcer. To evaluate the anti-ulcer, immunomodulating activities and toxicity of 50 and 100 °C water extracts, Vk50-I and Vk100-I respectively, from the roots of Vernonia kotschyana.
Characterization of extracts was carried out by GC, colorimetric and biological methods. Vk50-I and Vk100-I were administrated 50 min before induction of gastric ulcers in mice with 0.3 M HCl-60% EtOH. Inhibition of ulcer formation was calculated based on lesion index. Immunological activities were measured by complement fixation and macrophage activation. Toxicity assay was carried out on brine shrimps.
Vk50-I (98% inulin) and Vk100-I (83% inulin) from Vernonia kotschyana significantly inhibited the formation of gastric lesions in mice (100 mg/kg). No immunomodulating activities or toxicity were found.
Our results show that inulin is probably partly responsible for the anti-ulcer activity of Gastrosedal. In addition, it is possible that water soluble polysaccharides (mainly inulin) have an indirect impact on the general health of the GI.
来自 Vernonia kotschyana 的根在马里（西非）的基本药物国家清单上。它以 Gastrosedal 的名称出售，用于治疗胃炎和胃溃疡等疾病。为了评估 Vernonia kotschyana 根的 50°C 和 100°C 水提取物（分别为 Vk50-I 和 Vk100-I）的抗溃疡、免疫调节活性和毒性。
通过 GC、比色法和生物方法对提取物进行了表征。Vk50-I 和 Vk100-I 在小鼠用 0.3 M HCl-60%EtOH 诱导胃溃疡前 50 分钟给药。根据病变指数计算溃疡形成的抑制率。通过补体固定和巨噬细胞激活来测量免疫活性。在盐水虾上进行毒性测定。
来自 Vernonia kotschyana 的 Vk50-I（98%菊糖）和 Vk100-I（83%菊糖）显着抑制了小鼠胃损伤的形成（100mg/kg）。未发现免疫调节活性或毒性。
我们的结果表明，菊糖可能是 Gastrosedal 抗溃疡活性的部分原因。此外，水溶性多糖（主要是菊糖）可能对胃肠道的整体健康产生间接影响。
